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Clinical Microbiology Reviews, July 2002, p. 329-341, Vol. 15, No. 3
0893-8512/02/$04.00+0     DOI: 10.1128/CMR.15.3.329-341.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Methods for Cultivation of Luminal Parasitic Protists of Clinical Importance

C. Graham Clark1* and Louis S. Diamond2

Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, Great Britain,,1 Laboratory of Parasitic Diseases, National Institutes of Health, Bethesda, Maryland2

Cultivation of luminal protistan parasites has a long history. In this review we discuss the methods and media that are most widely used for the establishment and maintenance of the following organisms in culture: Entamoeba histolytica, Giardia intestinalis, Trichomonas vaginalis, Dientamoeba fragilis, Blastocystis hominis, and Balantidium coli. While cultivation is of limited importance in the diagnostic laboratory, it is essential to most research laboratories, and it is toward the latter that this review is primarily aimed.

* Corrresponding author. Mailing address: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St., London WC1E 7HT, England. Phone: 44-207-927-2351. Fax: 44-207-636-8739. E-mail: graham.clark{at}lshtm.ac.uk.

Clinical Microbiology Reviews, July 2002, p. 329-341, Vol. 15, No. 3
0893-8512/02/$04.00+0     DOI: 10.1128/CMR.15.3.329-341.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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