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Clinical Microbiology Reviews, January 2006, p. 127-141, Vol. 19, No. 1
0893-8512/06/$08.00+0     doi:10.1128/CMR.19.1.127-141.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Microbial Adhesion in Flow Displacement Systems

Henk J. Busscher and Henny C. van der Mei*

Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, 9700 AD Groningen, The Netherlands

Flow displacement systems are superior to many other (static) systems for studying microbial adhesion to surfaces because mass transport and prevailing shear conditions can be adequately controlled and notoriously ill-defined slight rinsing steps to remove so-called "loosely adhering organisms" can be avoided. In this review, we present the basic background required to calculate mass transport and shear rates in flow displacement systems, focusing on the parallel plate flow chamber as an example. Critical features in the design of flow displacement systems are discussed, as well as different strategies for data analysis. Finally, selected examples of working with flow displacement systems are given for diverse biomedical applications.


* Corresponding author. Mailing address: Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, P.O. Box 196, 9700 AD Groningen, The Netherlands. Phone: (31) 503633140. Fax: (31) 503633159. E-mail: h.c.van.der.mei{at}med.umcg.nl.


Clinical Microbiology Reviews, January 2006, p. 127-141, Vol. 19, No. 1
0893-8512/06/$08.00+0     doi:10.1128/CMR.19.1.127-141.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.




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