Clinical Microbiology Reviews, October 1998, p. 569-588, Vol. 11, No. 4
Department of Microbiology and Immunology,
College of Veterinary Medicine, Cornell University, Ithaca, New
York 14853-6401,1 and
Department of
Biochemistry and Immunology, Federal University of Minas Gerais,
Belo Horizonte 30161-970, MG, Brazil2
0893-8512/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Regulation and Function of T-Cell-Mediated Immunity
during Toxoplasma gondii Infection
SUMMARY
INTRODUCTION
INITIATION AND REGULATION OF T-CELL RESPONSES DURING
T. GONDII INFECTION
Role of Macrophages and Natural Killer Cells
Early T-Lymphocyte Activation
T lymphocytes.
T lymphocytes.
Modulation of T-cell activation.
Role of regulatory cytokines.
Role of nitric oxide.
EFFECTOR FUNCTIONS MEDIATED BY PARASITE-SPECIFIC
CD4+ AND CD8+ T CELLS
Parasite Antigen Entry into MHC Class I and II Pathways
of Presentation
Cytolytic T-Cell Activity
CD8+ CTL.
CD4+ CTL.
Importance of CTL activity versus IFN- production.
Production of Type 1 Cytokines
Protective Activities of Type 1 Cytokines
ROLE OF T-CELL-MEDIATED IMMUNITY IN THE PATHOLOGIC CHANGES
DUE TO TOXOPLASMOSIS
Acute Infection
Chronic Infection
HIV-Related Pathology
Ocular Toxoplasmosis
CONCLUSIONS AND FUTURE DIRECTIONS
ACKNOWLEDGMENTS
REFERENCES
SUMMARY
 Top NextReferences
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The intracellular protozoan Toxoplasma gondii is a widespread opportunistic parasite of humans and animals. Normally, T. gondii establishes itself within brain and skeletal muscle tissues, persisting for the life of the host. Initiating and sustaining strong T-cell-mediated immunity is crucial in preventing the emergence of T. gondii as a serious pathogen. The parasite induces high levels of gamma interferon (IFN-
) during initial infection as a result of early T-cell as well as natural killer (NK) cell activation. Induction of interleukin-12 by macrophages is a major mechanism driving early IFN- synthesis. The latter cytokine, in addition to promoting the differentiation of Th1 effectors, is important in macrophage activation and acquisition of microbicidal functions, such as nitric oxide release. During chronic infection, parasite-specific T lymphocytes release high levels of IFN-, which is required to prevent cyst reactivation. T-cell-mediated cytolytic activity against infected cells, while easily demonstrable, plays a secondary role to inflammatory cytokine production. While part of the clinical manifestations of toxoplasmosis results from direct tissue destruction by the parasite, inflammatory cytokine-mediated immunopathologic changes may also contribute to disease progression.
INTRODUCTION
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Life Cycle and General Aspects of Immunity
Toxoplasma gondii is an intracellular coccidian belonging to the phylum Apicomplexa. The parasite is globally distributed and can be found within many different species of mammals and birds. It is estimated that up to 5 × 108 people worldwide are infected with T. gondii. Sexual stages of the parasite occur within gut epithelial cells of the cat, and the products of gamete fusion, the oocysts, are shed in the feces. Once in contact with the atmosphere, the oocysts sporulate and become infective to other definitive or intermediate hosts. As in most coccidia, the sexual stages of Toxoplasma are highly specific, occurring in no other known hosts than those of feline species. Nevertheless, in contrast to other coccidia, Toxoplasma has evolved to infect a wide variety of vertebrate species, including humans. Indeed, this asexual stage of the parasite life cycle, unlike the sexual phase in cats, is notable for lack of both host and tissue specificity.
In the intermediate host, after infection of intestinal epithelial cells, the infective stages (oocysts or bradyzoites) transform into tachyzoites, which display rapid multiplication by endodyogeny within an intracellular parasitophorous vacuole. When the cells become packed with tachyzoites, the host cell plasma membrane ruptures and parasites are released into the extracellular milieu. The free tachyzoites can then infect virtually any nucleated cell they encounter, and they continue intracellular replication, spreading throughout host tissues (Fig. 1). If not controlled by the immune system, tachyzoites are highly virulent and cause a generalized toxoplasmosis which is always fatal (57). Indeed, many studies show that normally avirulent strains of T. gondii are highly virulent in T-lymphocyte-deficient animals (71, 133). Therefore, induction of T-cell-mediated immune responses and resistance to the tachyzoite stage is a key step in the T. gondii life cycle, determining the survival of the intermediate host and the parasite itself.
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After development of immunity, the tachyzoite stage is cleared from host tissues, and bradyzoites, the slowly multiplying, essentially dormant and harmless forms of the parasite, persist. The bradyzoites survive within cysts and are effectively isolated from the host immune system by the cyst wall, which is composed mainly of host tissue-derived products (Fig. 2A). The ability of bradyzoites to escape the host immune response and persist in a quiescent form within the host is therefore another key event in the T. gondii life cycle. The bradyzoites are infective for either definitive or intermediate hosts and are largely responsible for parasite transmission to different species of mammals and birds (Fig. 1).
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Although bradyzoites are apparently harmless, sequestered within dormant cysts, a persistent immunity to T. gondii is required to avoid reemergence of the tachyzoite stage and accompanying pathologic changes. Indeed, the latter is often observed in chronically infected immunocompromised hosts (139, 162, 213). Bradyzoites are found in proportionately larger numbers in the central nervous system (CNS); indeed, cyst reactivation most often occurs in the brain. This fact is well illustrated by the high incidence of encephalitis induced by T. gondii as a major cause of morbidity and mortality in patients with AIDS (139, 162).
Two hypotheses have been put forward regarding control of parasite replication during chronic toxoplasmosis. According to the first, the host immune response actively induces tachyzoite transformation into the bradyzoite stage and is crucial in maintaining T. gondii in the latter developmental form (Fig. 3A). Indeed, recent in vitro studies indicate that NO, an important effector molecule produced by activated macrophages, induces both parasite stasis and expression of a subset of bradyzoite-specific antigens (Ag) (14, 208).
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The second hypothesis suggests that an immune response controls
tachyzoite replication but does not, in itself, exert an effect on
bradyzoites, which are essentially harmless to the host (Fig. 3B).
Instead, it is suggested that parasites are continually released from
cysts in chronically infected hosts, resulting in constant boosting of
the immune system. Tachyzoite replication itself is controlled by the
immune system in immunocompetent animals. Strong evidence for this
model comes from the finding that in animals chronically infected with
T. gondii, treatment with neutralizing doses of antibody
(Ab) against either gamma interferon (IFN-) or tumor necrosis factor
alpha (TNF-
) results in the emergence of free tachyzoites and an
increased number of cysts (Fig. 2B and C).
It is noteworthy that in nature, mice are a major intermediate host of T. gondii. This would suggest that the mouse immune response is well adapted, in evolutionary terms, to cope optimally with this particular parasite. Of further note, the life cycle of the parasite in mice closely resembles that in humans. Toxoplasma is simple to maintain in vitro as tachyzoites and in vivo as either tachyzoites or bradyzoites. Thus, T. gondii infection in inbred mice has become a major model to elucidate the basis of protective immunity against intracellular pathogens in general and to examine the regulation of immunopathologic changes elicited by such infectious agents (62, 67, 200). The knowledge acquired from these studies has provided, and should continue to provide, new insights into designing more effective vaccines based on induction of cell-mediated immunity (CMI), as well as new strategies for immunotherapy of opportunistic infections in immunodeficient hosts.
Importance of T-Cell-Mediated Immunity in Resistance to T. gondii
One of the most distinctive immunologic features of T. gondii infection is the strong and persistent CMI elicited by the parasite, resulting in host protection against rapid tachyzoite growth and consequent pathologic changes. Another interesting aspect of Toxoplasma-triggered immunity is that it is normally harmless to the host. Thus, in contrast to many other parasites and several experimental models of toxoplasmosis, T. gondii under normal conditions fails to elicit significant immunopathologic changes in immunocompetent hosts and is usually accompanied by symptoms no more severe than fever, fatigue, and lymphadenopathy.
A series of early studies have pointed away from Ab and toward T cells as the major effectors of resistance to T. gondii. First, passive transfer of serum from chronically infected animals to naive recipients fails to protect the latter against virulent-parasite challenge (56). In addition, mice treated with antibodies against the µ heavy chain of immunoglobulin M (IgM) can control infection despite a lack of B lymphocytes and antibodies (177). Consistent with these observations is the fact that T. gondii lacks an extracellular stage, in contrast to some trypanosomatids such as Trypanosoma brucei, against which Ab have been shown to play a crucial protective role (127, 185). However, it is important to note that some studies indicate the importance of IgA as an element of mucosal immunity to oral infection with Toxoplasma cysts (30). Antibodies of this isotype may be important in avoiding reinfection with T. gondii, and therefore induction of IgA is a major strategy for vaccine development.
Studies showing the importance of T cells in resistance against T. gondii are nonequivocal. Thus, athymic nude mice, which lack functional T cells, are extremely susceptible to both virulent and avirulent parasite strains (71, 133). More importantly, adoptive transfer of immune T cells (in particular the CD8+ subset) to naive mice protects animals against challenge with virulent T. gondii strains (56, 168, 220, 221). Immunogenetic studies also point to a major influence of major histocompatibility complex (MHC) class I and II on resistance and susceptibility to the parasite, consistent with the idea that T lymphocytes are crucial in determining the outcome of infection (15, 16, 147, 149).
Virtually all mouse strains develop a strong Th1 immune response to
T. gondii, regardless of whether they possess resistant or
susceptible MHC haplotypes (61, 70). Thus, cytokines such as
IFN- and TNF- (which activate macrophage functions) are important for controlling tachyzoite replication during both acute and chronic phases of infection (61, 68, 110, 192, 213). In contrast, interleukin-10 (IL-10) and IL-12 appear to be crucial at the initial phase of infection and less important during chronic toxoplasmosis (75, 76).
While IL-12 is clearly important in initiating a strong and effective
CMI against T. gondii tachyzoites, IL-10 appears to modulate
both IL-12 and IFN- synthesis in vivo, avoiding an excessive immune
response that could cause extensive inflammation and host tissue damage
(76, 163). Thus, IL-10 and IL-12 are two major antagonists
involved in regulating IFN- synthesis during the initial phase of
infection. Whereas NK cells and CD4+ and CD8+ T
lymphocytes appear to be major sources of IFN- at the early stages
of infection 
T lymphocytes are the dominant source of this
cytokine during the chronic phase (58, 61, 70, 71, 75, 99, 117,
210).
In this paper, we review studies that have elucidated the molecular and cellular basis underlying the induction of T-cell-mediated immunity to T. gondii. We also discuss the effector mechanisms of parasite-triggered immunity, examining both beneficial and detrimental host effects that are brought into play during the encounter of this opportunistic pathogen with its mammalian host.
INITIATION AND REGULATION OF T-CELL RESPONSES DURING T. GONDII INFECTION
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Role of Macrophages and Natural Killer Cells
In addition to inducing an effective parasite-specific
T-cell-mediated immune response, it is important to note that T. gondii infection elicits strong nonspecific, T-cell-independent
immunity. Indeed, the latter response plays an important role in
influencing the development of parasite-specific T cells. The
nonspecific aspect is revealed by studies showing that T. gondii infection limits coinfection by nonrelated pathogens such
as Listeria monocytogenes and Schistosoma
mansoni, infection with certain viruses, and development of
certain tumors (69, 90, 143, 183). In addition, early studies demonstrated the ability of T. gondii to activate
cells from the innate compartment of the immune system, such as
macrophages and NK cells (85, 86). Further studies
demonstrated that nonspecific activation occurs at early stages of
infection, and is a T-cell-independent phenomenon resulting in IFN-
synthesis by NK cells and leading to macrophage microbicidal function
(62, 203). This early activation of the immune system
appears to play two main roles during infection with T. gondii. The first is to limit tachyzoite replication at a time
prior to recruitment of T-cell-mediated immunity. The second is to
direct the development of an appropriate T-cell response by driving the
differentiation of Th precursor (Thp) cells into Th1 effector cells.
These considerations suggest that T. gondii tachyzoites may
possess adjuvant-like activity that causes generalized potentiation of
CMI.
More recent studies have focused on elucidating the cytokine circuits,
host cell populations, and parasite molecules involved in the
adjuvanticity of tachyzoites. The use of mice with severe combined
immunodeficiency (scid mice), which lack both T and B cells,
and more recently the use of gene knockout (KO) mice have shed light on
these processes (8, 45). NK cells have been identified as a
major source of IFN- (203). The cytokine IL-12 appears to
be the central mediator initiating the synthesis of NK cell IFN-,
whereas other monokines, such as TNF-, IL-1 and IL-15, potentiate
the effects of IL-12 on NK cells (27, 71, 97, 100, 102, 105)
(Fig. 4A). A recent study also shows the importance of costimulatory molecules such as CD28 in stimulating IFN- synthesis by murine NK cells (102). A similar system
has recently been shown to be operative when human NK cells and
monocytes are cultured in the presence of soluble tachyzoite Ag (STAg)
(124).
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The above-described cytokine circuit appears to be functional in vivo
as well as in vitro. Thus, upon infection with T. gondii, scid mice produce high levels of IFN- and other
proinflammatory cytokines such as IL-12 (97). Similar
studies with scid mice show that in vivo neutralization of
IL-12 or IFN- enhances their susceptibility to acute T. gondii infection (75). Conversely, administration of
recombinant IL-12 prolongs the survival of scid animals, an
effect which is abolished by Ab-mediated NK cell or IFN- depletion
(71).
Enhanced susceptibility is also the outcome when immunocompetent mice
are treated at early stages of infection with anti-IL-12, anti-IFN-,
anti-TNF-, or anti-NK cell Ab (75, 99, 110). Furthermore,
in vivo treatment with either recombinant IL-12, IFN-, TNF-,
IL-1, or IL-1 has a protective effect against T. gondii infection (28, 71, 75). For the case of
recombinant IL-12 (rIL-12), protection was curtailed by treatment with
either anti-NK cell or anti-IFN- Ab. The latter finding suggests
that IL-12 may function through induction of NK cell IFN-; this
result finds support from the observation that IFN- KO mice are
susceptible to T. gondii despite maintaining an ability to
produce IL-12 (192).
The T-cell-independent system is potentiated by IL-2, and in certain
immunodeficiency states, this is critical to host survival. Thus, in
2-microglobulin KO animals, which lack CD8+
T lymphocytes, an enormous expansion of NK cells occurs in response to
infection, and these cells are responsible for controlling tachyzoite
proliferation via synthesis of IFN- (43). This NK cell
expansion appears to be dependent on CD4+ lymphocytes,
probably through the production of IL-2 itself (209). More
importantly, continuous treatment of AIDS patients with IL-2 leads to
enhancement of NK cell IFN- synthesis, compensating (at least in
part) for HIV-induced CD4+ T-lymphocyte deficiency
(124).
The early burst of IFN- and other proinflammatory cytokines is
crucial in shaping the adaptive immune response which develops against
T. gondii (67). First, IFN- triggers, or
potentiates, the synthesis of chemokines such as the macrophage gene
induced by IFN- (MIG) and IFN--inducible protein 10, which are
important in T-lymphocyte recruitment (1, 62). In addition,
IFN- synergizes with IL-12 in driving the differentiation of Thp
cells toward the Th1 phenotype (Fig. 4B). Thus, IFN- enhances IL-12
synthesis by macrophages exposed to tachyzoite products (71, 75,
141), induces expression of the IL-12 receptor on T cells
(108), and inhibits IL-4, an important cytokine for
differentiation of Thp to the Th2 phenotype (197).
Engagement of the IL-12 receptor also activates the phosphorylation of
signal transducer and activator of transcription 4 (STAT-4), a
transcription factor involved in Th1 lymphocyte differentiation
(6, 108, 197).
Interestingly, the Bcl-3 oncoprotein, a member of the I
B family
which functions as a positive regulator of NF-B activity, plays a
critical role in the development of T-cell-mediated immunity and
resistance to T. gondii (55). Most Bcl-3 KO mice
infected with T. gondii do not survive beyond 1 month,
whereas 100% of infected wild-type or heterozygote animals survive for
over 6 months. While STAg-stimulated splenocytes (or purified T cells) from Bcl-3 KO mice produce normal levels of IFN- during early (7 days) infection, the ability to produce this cytokine (after 12 days of
infection) is impaired. Cytotoxic T-lymphocyte (CTL) activity against
infected target cells, but not NK cytolytic activity, is also defective
in these animals. Together, these findings suggest that the Bcl-3 KO
mice possess an intrinsic T-cell defect responsible for blocking the
development of Th1 cells, resulting in compromised adaptive, rather
than natural, immunity to T. gondii.
Because of the importance of IL-12 in the development of CMI, several
groups are characterizing signalling pathways involved in IL-12
induction by microbial products. Briefly, IL-12 is a heterodimer
cytokine composed of two chains, p35 and p40, linked by a disulfide
bond. Whereas the p40 chain is tightly regulated in macrophages and can
be induced with different microbial stimuli, the p35 chain is
constitutively expressed by many different types of cells and tissues
(12, 225). The signalling pathways involved in the induction
of the p40 chain have been partially characterized. First, several
studies have demonstrated the requirement of two signals for optimal
IL-12 (p40) synthesis by macrophages. The first is a priming signal
provided by IFN-, and the second comes from microbial products
themselves, such as lipopolysaccharide or STAg (58, 71, 75,
141).
Recent studies have identified the IFN- consensus sequence binding
protein (ICSBP) as an important element in IL-12(p40) responsiveness to
IFN- (95, 189). The ICSBP gene is normally induced by
IFN-, and mice lacking a functional ICSBP gene are highly deficient
in IL-12 synthesis both in vivo and in vitro, although they produce
normal levels of IL-12- independent IFN- upon mitogen stimulation.
More importantly, the ICSBP KO animals are highly susceptible to
T. gondii, although they express normal levels of other
proinflammatory cytokines, such as IL-1, TNF-, and IL-6
(189). This result indicates that ICSBP activity is required
for IL-12 production exclusively. In contrast, activation of
reactivating kinase (or p38) from the mitogen-activated protein kinase-activated protein 2 pathway appears to be necessary for the
induction of several monokines, including IL-1, TNF-, and IL-12(p40)
itself (11, 22, 129). Molecular analysis of the IL-12(p40)
gene promoter suggests a functional role for an NF-B half-site, a
nucleotide sequence matched at 8 of 10 nucleotides forming the
consensus NF-B sequence (141). In addition, the ets-2
element is present in the promoter region (156). The ets molecules form a large family of transcription factors, some of which
are known to be important for the regulation of other inflammatory cytokine genes, such as TNF-. The induction of IL-12(p40) is modulated by cyclic AMP (22), consistent with early reports showing suppressive effects of prostaglandins on expression of this
cytokine.
A central paradox has been raised by these experiments: If IFN- is
required for the induction of macrophage IL-12 synthesis and if IFN-
production requires IL-12, which of these cytokines is produced first
during infection? Of relevance to this issue, it has recently been
found that mouse dendritic cells produce high levels of IL-12 in
response to T. gondii stimulation, even in the absence of
IFN- (175). In addition, STAg-induced IL-12 synthesis
occurs independently of CD40-CD40L interactions. This observation
suggests lack of a requirement for T-cell signalling to dendritic cells
through CD40-CD40L interaction (175), the latter of which
has been shown to be necessary in several other systems (39, 198,
204). However, as mentioned above, mice without a functional
ICSBP gene are deficient in IL-12 synthesis and are highly susceptible
to T. gondii infection, although their macrophage effector
functions remain normal (189). Thus, this study suggests
that IFN- is necessary for optimal production of IL-12 and
development of CMI in vivo. It is also important to note that in
contrast to Th precursor cells, resting NK cells express the IL-12
receptor and can therefore respond to IL-12 even in the absence of
IFN-.
Thus, during T-cell differentiation, it is likely that the NK cells
provide the initial IFN- source, which functions to further enhance
IL-12 synthesis initiated by microbial stimuli. Nevertheless, recent
evidence also indicates that Toxoplasma displays the
capability of activating T cells in an IL-12-independent manner (Fig.
4C) (191). This may also relate to studies in humans and mice
(described below), which suggest that the parasite can act as a
T-cell-specific and possibly a V-specific mitogen (41,
211).
Thus, during the earliest stages of infection, Toxoplasma
triggers several components of the innate immune system (Fig. 4). Macrophages, NK cells, and dendritic cells and neutrophils (144, 175) respond by releasing cytokines such as IL-12, TNF-, and IFN-. Within this milieu of proinflammatory cytokines, components of
the acquired immune system important for control of infection, namely,
T lymphocytes, recognize the appropriate combination of parasite Ag,
MHC, and costimulatory molecules. Viewed in this regard, it is clear
that innate immune responses exert a strong influence on developing
T-cell immunity, and it should not be surprising that T. gondii drives an extremely powerful Th1 response.
Early T-Lymphocyte Activation
During the early stages of Toxoplasma infection, as
described above, macrophages and NK cells become activated and produce cytokines, such as IL-12, which drive the development of a strong T-cell-mediated response. An additional component of the
above-described response is the ability of T. gondii to
induce T-lymphocyte proliferation and IFN- production during early
acute infection. Studies in both humans and mice reveal that both
and T lymphocytes display functional activity during the
first days of infection, which may also contribute to the adjuvant
properties observed during infection with the parasite. The molecular
basis for how T. gondii drives such a large initial T-cell
response is not known, but it may relate in part to superantigen-like
properties displayed by tachyzoites in vitro.
T lymphocytes.
Through the use of T-cell
receptor (TCR) KO mice and depleting monoclonal Ab mAb, T cells
are now known to play a protective role against several bacterial and
protozoan pathogens (32, 118, 150, 151, 182, 226). The
protective effects of T cells are often (although not always
[25, 226]) associated with early disease stages rather
than later in infection or during reinfection models. Indeed,
-T-cell numbers are elevated during early infection with
bacterial pathogens such as Listeria monocytogenes (165). These findings have led to the general view that
T cells find their major role as members of the armamentarium
providing the "first line of defense" against infection.
T
lymphocytes have been detected in the spleen and peritoneal cavity
within 7 days of infection with PLK and Beverley parasite strains, and the cells secrete IFN- and TNF- in response to in vitro
stimulation with the parasite (91, 93, 117). As such,
T cells may play a role in early macrophage activation; indeed, in vivo
MAb-mediated T cell depletion results in depressed levels of
macrophage NO production (93). The T lymphocytes
display protective activity, as demonstrated by adoptive transfer, MAb
depletion, and TCR KO experiments (91, 117).
Elevated T-lymphocyte levels also occur in humans with acute
toxoplasmosis. In these cases, preferential expansion of
V2+ T cells has been reported (49,
188). In patients with acute congenital toxoplasmosis,
V2+ cells display nonresponsiveness to in vitro
stimulation with either parasite or anti-CD3 stimulation in vitro, as
do T cells (84). While V2+ function is
regained during later infection, as measured by proliferation and
IFN- secretion, T cells remain largely nonresponsive. These
data raise the novel possibility that T lymphocytes contribute to protection during chronic stages of human congenital infection.
In vitro studies indicate that parasite stimulation of peripheral blood
T cells induces preferential V9+
V2+ T-lymphocyte expansion (210). The latter
population produces IFN-, IL-2, and TNF- and displays
MHC-unrestricted cytotoxicity toward infected target cells. T cells
expressing the same V9 TCR component respond to other pathogens such
as Plasmodium falciparum and Mycobacterium
tuberculosis (78, 112). With regard to Ag specificity,
V9+ V2+ T lymphocytes recognize a series
of nonprotein low-molecular-weight mycobacterial and synthetic
compounds (32, 34, 194, 224). These simple molecules, some
of which are phosphorylated metabolites, are widely expressed by
eukaryotic cells, raising the possibility that similar
cell-stimulating molecules are expressed by T. gondii.
Interestingly, T cells have also been implicated in the
induction of macrophage heat shock protein, possibly through IFN- production, during early T. gondii infection. Thus,
treatment with anti- T-cell MAb blocks hsp65 induction during
infection with the Beverley strain (91, 159). Expression of
hsp65 correlates with protection against T. gondii
(160). The significance of the latter finding can possibly
be explained by recent results suggesting that hsp65 induction prevents
macrophages from undergoing a programmed cell death response after
parasite infection (92). Thus, it is noteworthy that the
highly virulent strain, RH, fails to induce macrophage hsp65, and host
cells undergo apoptosis, whereas infection with the low-virulence
strain, Beverley, induces hsp65 and triggering for programmed cell
death does not occur. A causal linkage between expression of hsp65 and
prevention of apoptosis was suggested by the finding that an hsp65
antisense probe inhibited the relative proportion of cells undergoing
programmed cell death (92).
Thus, T lymphocytes expressing the TCR appear to be important
during Toxoplasma infection. This is probably a result of their ability to produce type 1 cytokines which exert microbicidal effects on the parasite, as well as promoting the development of a
strong inflammatory cytokine response. Nevertheless, little is yet
known about the Ag specificity of this T-cell subset during T. gondii infection, nor is anything known about their requirement for recognizing processed peptide (or possibly nonpeptide) ligand in
the context of the MHC and other accessory molecules. These issues,
which are basic to the general biology of T lymphocytes, should
provide investigators with a challenging avenue of inquiry for several
years to come.
T lymphocytes.
Infection with T. gondii also provides a direct and potent stimulus for
T-lymphocyte activation, and as a result, T-cell-derived IFN-
production occurs early during acute infection. In part, early T-cell
production of IFN- may be explained by the ability of host
Ag-presenting cells to activate T lymphocytes in a milieu of
inflammatory cytokines. While such an early type 1 cytokine response
may be expected to serve a protective role for the host, under certain
conditions, T. gondii-induced inflammatory cytokines may
contribute to the pathologic findings of the disease.
-T-cell activation comes from a
variety of reports. -T-cell production of IFN- during acute infection (7 days postinoculation) has been linked to host protection during intraperitoneal infection with the cystogenic ME49 parasite strain (75). In addition, CD8+ intraepithelial
lymphocytes isolated 11 days after oral infection are able to
adoptively transfer protection in an IFN--dependent manner
(19). CD4-mediated pathologic findings have also been reported in 7-day orally infected C57BL/6 mice (131) and
during acute infection of IL-10 KO mice (discussed below) (76,
163).
While classical processing and presentation of antigenic peptide in the
context of MHC class I or II and the appropriate costimulatory molecules undoubtedly plays a major role in this early T-cell activity
(47), there is evidence to suggest that in mice, although not in humans, T. gondii possesses superantigen-like
properties that may contribute to early T-cell activation. Culture of
spleen cells from noninfected mice with tachyzoite lysate or irradiated parasites results in a vigorous T-cell proliferative response and
secretion of IFN-. Interestingly, in extended (7-day) cultures, CD8+ T lymphocytes preferentially expand in number relative
to CD4+ cells, and V5+ lymphocytes are
overrepresented among the T cells (41). In vivo studies also
suggest an early V5+-cell expansion during acute murine
toxoplasmosis (122). However, in the in vivo situation,
V5+ cells, although initially responding, become anergic
to ex vivo stimulation with parasite Ag or TCR MAb (40,
122).
The cellular basis of preferential CD8+ expansion in vitro
is not known, but several models can be invoked based on the following observations. First, as shown in Fig. 5,
coincubation of infected Ag-presenting cells with purified
CD4+ cells induces strong proliferation, demonstrating that
the latter cell type is not inherently nonresponsive to parasite
stimulation (42). Second, purified CD8+ T
lymphocytes do not proliferate in response to infected Ag-presenting cells unless supplied with exogenous IL-2 (Fig. 5). These results indicate that CD4+ cells may initially be triggered to
release IL-2, which helps drive CD8+ proliferation and
ultimately results in outgrowth of CD8+ T cells. In
addition to active suppression of CD4+ cells by
CD8+-derived lymphokines, it is possible that
CD4+, but not CD8+, T cells undergo
activation-induced cell death, as is reported to occur during
Trypanosoma cruzi infection (50). Such a
phenomenon may have as its basis divergent apoptosis-inducing signals
among CD4+- and CD8+-T-cell populations
(239). Interestingly, several reports indicate that during
human toxoplasmosis, CD8+ T cells increase in number
relative to CD4+ T lymphocytes
a finding which suggests a
clinical correlate to the in vitro murine studies (94, 140).
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secretion (211). However, unlike the situation in
mice, human T cells show no consistent TCR V skewing after in vitro stimulation. Moreover, while MHC class II is required in the response, paraformaldehyde fixation of the Ag-presenting cells prior to incubation with parasite Ag abrogates the activity, indicating a
requirement for Ag processing. Thus, present data suggests that although T. gondii is a powerful stimulator of early human
T-cell responses, unlike the case in mice, the human response resembles more a classical Ag-driven rather than a superantigen-driven
phenomenon.
Modulation of T-cell activation.
While early production
of inflammatory cytokines is required for the subsequent
parasite-induced protective T-cell-mediated immune response, if
uncontrolled this response can lead to severe immunopathologic changes
and even to death. Thus, under normal circumstances, the strength of
the host CMI triggered by T. gondii must be tightly
regulated to limit infection, on the one hand, and to avoid
immunopathologic changes, on the other. Elucidation of the molecular
mechanisms underlying regulation of the protective immune response has
provided important clues for understanding the basis of successful
long-term interaction between parasites and their vertebrate host. At
the same time that macrophages induce strong type 1 cytokines, these
cells also promote the regulation of CMI through production of IL-10
and transforming growth factor (TGF-), which regulate the
expression and function of IL-12 and other monokines (62). A
recent study also suggests that IL-4 may display a similar regulatory
role during acute toxoplasmosis (179). In addition, upon
stimulation with microbial products, macrophages produce high levels of
NO, which displays potent antiproliferative effects on cells from the
lymphocytic lineage (62).
Role of regulatory cytokines.
The cytokine IL-10 was
first identified by its ability to inhibit IFN- synthesis by Th1
lymphocytes and was shown to be produced by Th2 cells (53).
Similar effects of IL-10 were also observed on in vitro IFN-
synthesis by STAg-stimulated NK cells from scid mice
(203). Follow-up studies indicated that IL-10 is produced by
a large variety of cells in addition to Th2 lymphocytes, including B
cells and macrophages. Indeed, the latter cell population, itself, appears to be the main target of IL-10 suppressive action
(153). Thus, IL-10 inhibits the synthesis of a wide variety
of proinflammatory monokines by macrophages and is therefore an
important modulator of macrophage effector functions against different
pathogens, including T. gondii (54, 74). The main
method by which IL-10 inhibits IFN- synthesis by NK and Th1
lymphocytes is through inhibition of macrophage IL-12 synthesis
(37, 96).
in serum relative
to those in uninfected IL-10 KO or infected wild-type mice
(76). Importantly, although the parasite burden was similar
or decreased in the IL-10 KO mice, the animals displayed enhanced
pathologic changes in the liver and, to a lesser extent, the lung
tissue. Taken together, our results and similar studies by others
suggest that the increased mortality of IL-10 KO mice is due to an
abnormally high inflammatory cytokine response during acute
toxoplasmosis rather than to uncontrolled parasite replication
(76, 163). These findings indicate that IL-10 is an
important physiological regulator of proinflammatory cytokine induction
and that simultaneous induction of IL-10 during CMI initiation is of
crucial importance in avoiding an overwhelming type 1 cytokine
response.
The cytokine IL-4 has been previously shown to inhibit certain
macrophage functions and to potentiate the effect of IL-10 on
macrophages (166, 202). In addition, IL-4 plays a major role in controlling the development of CMI is through its effects on Thp
cells, resulting in STAT 6 induction and T-cell differentiation to the
Th2 phenotype (130, 174, 223). Thus, IL-4 KO mice are also
more susceptible to acute T. gondii infection than are
wild-type animals (179). Similar to the IL-10 KO studies,
the enhanced mortality in IL-4 KO mice is accompanied by increased
expression of IFN- and lower tissue parasitism. Although the
mechanism is not yet clear, this study also suggests that IL-4, by
potentiating IL-10 effects and/or antagonizing Thp-cell differentiation
toward the Th1 phenotype, is another important cytokine preventing the induction of an overwhelming parasite-induced Th1 response.
Nevertheless, it should be noted that a clear view on the role of IL-4
during toxoplasmosis has yet to emerge. While some studies suggest a
role for this cytokine in avoiding pathologic changes (as discussed
above), others suggest that absence of this mediator may be beneficial
to the host in surviving acute infection (228). Part of this
confusion may be due to the use of different parasite strains, and it
would also seem to indicate that the line between protective and
pathologic immune responses is easily crossed.
In addition to IL-4 and IL-10, TGF- is an important regulator of
macrophage activation (227). This cytokine has been shown to
influence macrophage effector function (e.g., NO production) against
several protozoan parasites (9, 207). Furthermore, TGF-
potentiates the effects of IL-10 on macrophages and inhibits IL-12-induced IFN- synthesis by NK cells (73, 98, 166). Interestingly, the latter activity appears to operate at the level of
inhibition of function, rather than synthesis, since in vivo treatment
with TGF- blocks the protective effects of rIL-12 during T. gondii infection in scid mice. While this study
suggests that by inhibiting NK-cell IFN- synthesis, TGF- may be a
potent regulator of CMI development, this hypothesis has not yet been
tested in immunocompetent mice. Nevertheless, studies with the
Leishmania model show that in vivo treatment of genetically
resistant mice with TGF- favors Thp-cell differentiation toward the
Th2 instead of the expected Th1 phenotype, resulting in enhanced
susceptibility to the parasite (9). Therefore, several
independent studies indicate that TGF- treatment enhances
susceptibility to infection with several distinct microbial pathogens
by regulating the development of protective CMI.
Role of nitric oxide.
Another important mechanism by
which macrophages regulate the immune response during experimental
acute toxoplasmosis is through generation of NO (109). After
priming with IFN-, macrophages exposed to microbial products or
TNF- produce high levels of reactive nitrogen intermediates (RNI).
These compounds were first recognized for their importance as effector
molecules responsible for microbicidal and microbiostatic functions
displayed by activated murine macrophages (see below). Experiments
performed both in vivo and in vitro also reveal an immunosuppressive
activity associated with RNI, in particular during the early phase of
infection with T. gondii (23).
synthesis is only partially suppressed (87). Interestingly, this
CD4+-T-cell-suppressive effect is macrophage mediated and
is inhibited by neutralization of either endogenous TNF- or IFN-.
Both cytokines are required for induction of optimal levels of
inducible NO synthase (iNOS) and RNI (109). In addition,
synthetic inhibitors of iNOS partially overcome this immunosuppressive
effect (23, 87). The importance of NO as an immunoregulatory
molecule is supported by findings in animals infected with T. gondii and treated with iNOS inhibitors (87). Thus,
treatment with these synthetic compounds results in increased cyst
numbers and an intensified inflammatory reaction in the CNS. Although
not completely understood, recent studies indicate that the regulatory
effects of RNI on T lymphocytes is due at least in part to the
induction of apoptosis (145).
Recent findings in Toxoplasma-infected iNOS KO mice are
relevant to these studies. Thus, orally infected iNOS KO mice survive early infection with decreased inflammation in the small intestine and
increased survival relative to wild-type controls (123). This result suggests that some of the pathologic changes associated with acute infection involve NO-mediated effects. Indeed, these findings are reiterated in a model of T. gondii tachyzoite
Ag toxicity in D-galactosamine-sensitized mice, where the
lethality of the pathogen is associated with high NO levels in serum
(144).
Nevertheless, iNOS KO animals succumb during chronic infection with
increased parasite burden and inflammation in the brain (123,
190). Thus, in chronic infection, NO may act to down-regulate host pathologic changes that would otherwise be induced by presence of
the parasite. Therefore, our view is that although parasite-induced RNI
during acute infection can be detrimental, as the host enters the
chronic stage of disease, NO emerges as an important regulatory molecule involved in minimizing the immunopathologic changes induced by
the parasite.
EFFECTOR FUNCTIONS MEDIATED BY PARASITE-SPECIFIC CD4+ AND CD8+ T CELLS
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Acquired immunity induced by T. gondii is characterized
by strong CD4+ and CD8+ activity. Thus, through
infection of cells, or Ag shedding, parasite peptides are efficiently
presented to parasite-specific T lymphocytes. The lymphocytes that
differentiate in the environment of peptide Ag and type 1 cytokines
display CTL activity (in particular with respect to CD8+
effectors) and the ability to produce large amounts of IFN-. Production of the latter cytokine and other proinflammatory mediators is necessary for immunity, but evidence suggests that such cytokines must be tightly regulated to avoid pathologic changes.
Parasite Antigen Entry into MHC Class I and II Pathways of Presentation
Toxoplasma is a strong inducer of Ag-specific
CD4+ and CD8+ T lymphocytes, indicating that
parasite peptides are efficiently targeted to the appropriate cellular
pathways of Ag presentation during infection. In general, processing
for MHC class I presentation requires Ag entry into the cytoplasm,
followed by proteolytic generation of peptides by proteasomes,
transporter associated with antigen presentation-mediated transport
across the endoplasmic reticulum, association with MHC class I heavy
chain and 2-microglobulin, and exocytosis to the cell
surface (237). Presentation in the context of MHC class II
molecules generally requires soluble Ag endocytosis, proteolysis within
the phagolysosome, trafficking to MHC class II-containing endosomes,
association with MHC class II, and transport to the cell surface
(35, 232).
For T. gondii Ag, it is not clear where peptide loading onto MHC occurs. In one model, Ag-MHC interaction could occur at the cell surface, as a result of either Ag secretion by extracellular tachyzoites or deposition on the cell surface during invasion. This model would require proteolytic degradation of parasite Ag outside of host cells and would additionally require that parasite Ag displace already bound peptide. In an alternate model, presentation of parasite Ag would depend upon phagocytosis or receptor-mediated uptake of dead or dying tachyzoites or soluble parasite Ag. Parasites entering the cell in this manner are trafficked through the endosomal-lysomal pathway. Although this route classically favors MHC class II presentation, it is now clear that peptides can access the cytosolic presentation pathway by this route (111, 154). In this regard, murine bone marrow macrophages are extremely efficient at processing and presenting to soluble Ag cytolytic CD8+ T cells in a class I-restricted manner (42, 47).
A final model would involve the transport of Ag from within the parasitophorous vacuole to the cytosolic and possibly endocytic presentation pathway. The parasitophorous vacuole is believed to function as a molecular sieve, allowing free diffusion of molecules smaller than 1,300 to 1,900 Da between the host cytoplasm and parasitophorous vacuolar space (196). This property alone would exclude macromolecular Ag from accessing the host cell cytoplasm but instead suggests that antigenic peptides are formed within the parasitophorous vacuole and that this is followed by passive diffusion into the host cell cytoplasm. Nevertheless, intact antigenic proteins could also potentially be actively transported across the parasitophorous vacuole membrane into the host cell cytoplasm for subsequent trafficking to the conventional MHC presentation pathways.
Cytolytic T-Cell Activity
Generation of T lymphocytes possessing parasite-specific cytolytic
activity is a characteristic of both human and murine infection. In
humans, both CD4+ and CD8+ T cells with
cytolytic activity have been isolated from seropositive donors. For the
most part, studies with mice provide evidence for only CD8+
cytolytic activity. The ability of these T-cell subsets to
simultaneously produce the protective cytokine IFN- and exhibit
cytolytic function has obscured the issue of whether the latter
activity plays a role during infection. Nevertheless, the finding that
CD8+, but not CD4+, T cells are more efficient
at transferring immunity has led to the suggestion that CTL activity
contributes to protection. However, recent studies in mice defective
for cytolysis suggest that this function plays at best a secondary role
with respect to IFN- production.
CD8+ CTL. CTL are best known for their ability to kill virus-infected and transformed target cells. It is now clear that several intracellular protozoans are also effective at stimulating CD8+ CTL function against infected target cells, in part through their ability to traffic antigenic peptides into the MHC class I presentation pathway. Vaccination of mice with Plasmodium berghei and P. falciparum sporozoites generates circumsporozoite-specific CD8+ T cells capable of killing Ag-specific target cells and transferring protection to naive recipients (128, 181). Infection with Trypanosoma cruzi likewise results in the presentation of parasite-derived peptide with cell surface MHC class I glycoproteins, in turn inducing CD8+ CTL effector function (59, 164). The ability of these parasites to stimulate CTL function is most probably related to their capacity of evading the lysomal pathway during cell invasion, residing instead either directly in the cytoplasm (T. cruzi) or within a parasitophorous vacuole (Plasmodium). This may also explain why Leishmania spp., which find their niche within the macrophage phagolysosome, are relatively poor at inducing CD8+ CTL function (167, 230).
For the case of T. gondii, injection of the attenuated strain, ts-4, generates CD8+-T-cell effectors in mice. Together with CD4+ T lymphocytes, these cells confer strong protective immunity to subsequent challenge with the highly virulent RH strain (66). In addition to releasing IFN- in response to
parasite Ag, CD8+ cells display strong MHC class
I-restricted CTL activity toward infected target cells (42, 82,
212). Oral infection also results in a population of CD8/
TCR-positive intraepithelial lymphocytes which are capable of
transferring protection and which display the in vitro activities of
IFN- secretion and cytolytic activity toward infected enterocytes
(19, 29). HLA class I-restricted CD8+ CTL
activity has been found in the peripheral blood of humans with acute
toxoplasmosis, and CD8+ CTL clones can be cultured from
peripheral blood lymphocytes of chronically infected patients
(152, 173, 234). Thus, in both humans and mice,
Toxoplasma infection provides a potent stimulus for the
generation of CD8+ effectors capable of lysing
parasite-infected target cells.
Experiments in 2-microglobulin KO mice, which lack
peripheral CD8+ T lymphocytes, reveal that absence of the
latter effectors, or possibly lack of MHC class I expression itself,
drives preferential expansion of NK cells in response to ts-4
vaccination (43, 45). This result, which is also found when
the class I KO strain is infected with lymphocytic choriomeningitis
virus (209), underscores the importance of CD8+
effector cells in the normal host response to infection. Thus, when
class I Ag-presenting cells are crippled by inactivation of
2-microglobulin, the murine host responds with a
remarkable overproduction of NK cells, which provide partial protection
through IFN- production (43). Nevertheless, we have also
found that 2-microglobulin-deficient mice, while
surviving acute infection, succumb during chronic infection with the
cystogenic Toxoplasma strain, ME49 (Fig.
6).
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in
response to SAG-1 in vitro (116, 119). Moreover, the clones
are capable of conferring immunity to challenge infection in adoptive
transfer experiments (119).
Although SAG-1 can elicit protective CD8 responses when presented in
the context of MHC class I, it is clear that other parasite proteins
are also recognized by CD8+ cells during infection. In this
regard, when soluble tachyzoite extract is subjected to biochemical
fractionation, the major cytolytic activity is contained within a
fraction which does not include the SAG-1 protein when tested with
splenocyte effectors from Toxoplasma-immune mice
(42). In addition, peptides which sensitize human targets for CTL lysis have been isolated by acid elution from a T. gondii-infected B-cell lymphoma (3). Amino acid
analysis of the peptides reveals that they are not derived from protein
of any of the cloned genes of the parasite, including SAG-1. Thus,
while SAG-1 can act as a target of CD8+ T cells during
infection, other T. gondii Ag are likewise able to elicit
CTL responses. The identity of these other Ag is, as yet, unknown.
Generation of CD8+ effectors requires the presence of
CD4+ T lymphocytes during vaccination with ts-4, since
removal of the latter cell type with depleting MAb results in failure
to generate protective CD8 activity (70) and since removal
of CD4+ cells abrogates Ag-driven CD8 proliferation (Fig.
5). Nevertheless, MHC class II (A) KO mice, which lack class
II-restricted CD4+ T cells, maintain the ability to
generate CD8 effector cells displaying CTL activity and IFN-
production. The resolution to this apparent paradox is that a
population of CD4+ NK1.1+ (NK1) T cells
provides helper function for CD8 generation (44, 46). This
novel T-cell subpopulation functions independently of MHC class II but
is selected during thymic ontogeny by the class I-like molecule, CD1
(10). NK1 T cells produce IL-2 early during the period of
ts-4 vaccination, and this activity provides an essential helper
activity for generation of CD8 effector cells.
Nevertheless, although A KO mice survive acute ME49 infection, the
animals succumb during early chronic infection (Fig. 6). Possibly, the
reason why the KO animals survive acute infection is that
CD8+ effectors, generated through NK1 T-cell IL-2
production, provide protection during acute infection. However, during
chronic infection, both CD8+ and conventional MHC class
II-restricted CD4+ T cells are required to prevent cyst
reactivation (61), and in the absence of the latter
population, the A KO strain would fail to survive.
NK1 T cells have gained considerable attention recently because of
their ability to release large amounts of cytokines, in particular
IL-4, without the need for priming Ag. As such, these cells are
believed to be positioned at the earliest stages of immune response
induction and may be important in influencing the pattern of cytokines
that subsequently develops (10). It is becoming increasingly
clear that NK1 T cells are capable of producing other cytokines, such
as IFN-, when appropriately stimulated (31). The data
from the T. gondii studies show that IL-2 secretion is also
an important functional activity of these cells.
CD4+ CTL.
While CD4+
lymphocytes are not generally considered major cytotoxic effectors,
several reports indicate that Toxoplasma infection in humans
results in generation of CTL of the helper T-cell phenotype (24,
36, 152, 173). Indeed, some groups report that CD4+
CTL are easier to isolate in vitro than CD8+ CTL, although
the explanation for this phenomenon is presently unknown
(173). Interestingly, human CTL lines display a predominant V7 TCR usage, possibly indicating the presence of an immunodominant Ag (152).
-secreting, DPw4-restricted clone was generated which possesses specificity for the rhoptry 2 (ROP-2) protein of T. gondii
(88, 187). The ROP-2 protein itself possess three potential
T-cell epitopes as predicted by computer algorithms (186).
When these peptides were synthesized and tested in vitro, it was found
T cells from a large proportion of donors seropositive for
Toxoplasma responded to at least one synthetic peptide. The
ROP-2 protein is therefore likely to be a major Ag recognized during
the human T-lymphocyte response to the parasite.
Importance of CTL activity versus IFN- production.
Infection with T. gondii provides a strong stimulus for
CTL activity in mice (with respect to CD8+ T cells) and
humans (with respect to both CD8+ and CD4+
lymphocytes). Nevertheless, these cells are also well known for their
ability to simultaneously produce high levels of IFN- in response to
the parasite. The requirement for the latter cytokine in immunity to
T. gondii (see below) has made it difficult to evaluate the
contribution of CTL activity in resistance and susceptibility to the
parasite.
KO mice
(45, 48, 192). Therefore, perforin-mediated CTL activity
does not appear to be required for resistance to acute infection. While
it is possible that other cytolytic mechanisms are involved (e.g.,
Fas/Fas ligand- and TNF--triggered cell death [115, 137,
239]), this seems unlikely since no cytolytic activity can be
detected in the perforin KO strain, and, indeed, perforin-dependent cytolysis is generally considered the major mechanism of CTL activity (33, 171).
Despite the finding that control of acute infection does not require
CTL activity, the perforin KO strain is slightly more susceptible than
wild-type animals during chronic ME49 infection (48). Thus,
the KO mice harbor approximately three times the number of cysts as
wild-type mice, and the animals succumb to long-term chronic infection
at a slightly accelerated rate. Therefore, CTL function appears to play
a role in host protection during the later stages of infection,
possibly contributing to the prevention of cyst reactivation or,
alternatively, limiting the number of parasites initially encysting
within tissues of the CNS.
Nevertheless, ME49-infected CD8 KO (48) and
2-microglobulin KO (Fig. 6) animals die much more
rapidly during chronic infection than do perforin-deficient mice;
indeed, the absolute requirement for IFN- to survive chronic
infection is well established (61, 192, 213). Together,
these findings confirm the importance of CD8+ T cells
during infection, and they establish that although CTL activity can
contribute to control of infection, the ability to produce IFN- is
most probably the key characteristic of this major effector population
(Fig. 7).
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Production of Type 1 Cytokines
As discussed above, infection with T. gondii induces
strong CMI, characterized by a highly polarized Th1-cell response
(62, 67, 200). The acute phase of infection is marked by
elevated levels of IFN- and IL-12, as well as other proinflammatory
cytokines such as TNF-, granulocyte-macrophage colony-stimulating
factor, IL-6, and IL-1. Nevertheless, IL-10, an anti-inflammatory
cytokine, is also induced at this stage of infection. Macrophages
and/or dendritic cells are generally considered the major source of
proinflammatory cytokines, as well as the anti-inflammatory mediator
IL-10, during acute T. gondii infection. In addition,
neutrophils, like macrophages, are capable of producing both
proinflammatory and anti-inflammatory cytokines during early infection
(144, 180). In the first days following inoculation with
T. gondii, NK cells and T lymphocytes provide a major source
of IFN-. Perhaps not surprisingly, given the cross-regulatory
properties of type 1 and type 2 cytokines (199), infection
is associated with low levels of IL-4 and IL-5 (75).
As the chronic stage of infection progresses in mice, levels of
proinflammatory cytokines such as IL-1, IL-6, TNF-, and IFN- decrease (as measured by presence of gene transcripts in the brain) while the level of the anti-inflammatory cytokine IL-10 increases (68). Interestingly, IL-4 can also be briefly detected early in chronic disease (days 10 to 15 after infection), but its expression rapidly falls to background levels (103). During the chronic phase, both CD4+ and CD8+ T lymphocytes are
required to prevent reactivation of toxoplasmosis (61). When
the latter cells are stimulated in vitro with parasite Ag, they produce
high levels of both IFN- and IL-2. Unlike acute-stage disease, NK
cells do not appear to contribute significantly to cytokine production
during the persistant period of infection (75). This general
pattern of a polarized type 1 cytokine response associated with chronic
toxoplasmosis has also been reported in human patients (63,
142).
In susceptible mouse strains (such as C57BL/6), a moderate inflammation
characterized by the presence of gene transcripts for IFN-, TNF-,
granulocyte-macrophage colony-stimulating factor, IL-6, IL-1, and
IL-10, is found in the CNS of chronically infected animals (68,
103, 104). Interestingly, the inflammatory infiltrate appears to
be dominated largely by CD8+ and CD4+ T
lymphocytes (101, 193). The moderate inflammatory process may gradually increase in severity (depending upon the mouse strain and
parasite dose), resulting in a high rate of mortality over a period of
2 to 6 months. Both in vitro and in vivo studies show that cytokines
such as IFN- and TNF- are key mediators in triggering the
effector functions against T. gondii during both acute and chronic stages of infection. However, if uncontrolled, this T. gondii-induced CMI response can lead to host tissue damage,
pathologic changes, and, sometimes, death.
Protective Activities of Type 1 Cytokines
Cytokines produced by T lymphocytes, such as IL-2, IFN- and
TNF, trigger important effector mechanisms mediated by other cells of
the immune system. As described above, IL-2 produced by
CD4+ T lymphocytes is an important growth factor for
generating CD8+-lymphocyte effector functions, namely, CTL
activity and IFN- production. In addition, IL-2 can enhance NK cell
expansion, lytic activity, and IFN- synthesis initiated by IL-12
(38, 125).
The cytokine IFN- is central in resistance to T. gondii
at both acute and chronic stages of infection, as demonstrated by cytokine depletion, cytokine repletion, and gene knockout studies (70, 192, 214, 218). Although IFN- may play multiple
roles in resistance to the parasite, macrophage activation is generally believed to be the critical effector function (2, 62). As discussed above, macrophage activation results in iNOS gene induction and synthesis of moderate levels of RNI (109). In general,
RNI are produced as by-products during degradation of arginine into citrulline by iNOS. Synthesis of RNI is highly potentiated by certain
microbial products, as well as by TNF-. Indeed, pathogen-derived factors themselves may enhance RNI production indirectly, by triggering macrophage TNF- synthesis (109). Nevertheless, results
with T. gondii-infected TNF receptor KO mice, showing that
NO levels are normal, suggest that the requirement for TNF-triggering
is not absolute (235).
In vivo experiments are highly consistent with a role for inflammatory
cytokines in resistance to T. gondii. Thus, MAb
neutralization of endogenous IL-12, TNF-, or IFN- leads to 100%
mortality during the acute phase of infection with an avirulent
parasite strain (75, 110). Similarly, neutralization of
TNF- or IFN- leads to decreased CNS-associated iNOS gene
expression, reactivation of chronic toxoplasmosis, and death due to
toxoplasmic encephalitis (TE) (68).
The importance of iNOS activity in resistance to T. gondii
has recently been confirmed by infecting iNOS KO mice with an avirulent strain of T. gondii. At approximately 21 to 30 days
postinfection, iNOS KO mice begin to succumb to severe TE associated
with large numbers of cysts and tachyzoites, a result demonstrating the
importance of endogenous NO in the control of chronic infection
(190). Consistent with this finding are experiments with
animals lacking the transcription factor IFN regulatory factor 1 (IRF-1), which is essential for IFN- iNOS induction
(121). Although the IRF-1 KO animals are more susceptible to
infection with T. gondii than are wild-type animals, they
maintain an early line of defense, which is independent of iNOS
activity.
While iNOS KO and IRF-1 KO mouse strains do not succumb until a time
normally associated with cyst formation and initiation of chronic
infection, neither IL-12 KO nor IFN- KO animals are capable of
surviving beyond 10 days after T. gondii infection (218). These results together make it clear that in addition to iNOS induction, other IFN--dependent mechanisms are important for
controlling tachyzoite replication at both the acute and chronic stages
of disease. Candidate mechanisms would be release of reactive oxygen
intermediates (ROI) (161), tryptophan degradation (157, 170), or a novel mechanism that has yet to be characterized.
The role of iNOS-induced RNI as an effector mechanism of human
macrophages is questionable (157, 158). However, in vitro studies indicate that IFN--induced tryptophan degradation, resulting in tachyzoite growth cessation, is an important antiparasite mechanism in both human macrophages and fibroblasts (157, 170).
Indeed, IFN- triggers the same degradative pathway in many other
cell types in both human and murine systems (89). The
release of ROI and generation of leukotrienes by IFN--activated
human macrophages has also been implicated in the control of tachyzoite
replication (134, 135, 161, 236). Nevertheless, while these
activities can exert a microbicidal or microbiostatic activity in
vitro, their functional significance during clinical toxoplasmosis is not known.
IFN- has other important effects on the immune system that may
contribute to resistance against T. gondii. Thus, IFN- is an important factor for CTL differentiation (238), as well
as for promotion of upregulated MHC expression (136). Both
activities would favor CTL effector function. Moreover, B-cell
synthesis of specific IgG isotypes, namely, IgG1 in humans and IgG2a in mice, is driven in part by IFN- (52). Indeed, the
parasite-specific humoral response during T. gondii
infection is dominated by IgG1 and IgG2a isotypes in humans and mice,
respectively (83, 106). These isotypes can play an important
role in resistance to different pathogens through mechanisms such as
complement fixation, opsonization, or Ab-dependent cell cytotoxicity.
Nevertheless, it seems unlikely that these IFN--influenced effects
on components of acquired immunity would be operative at the early time
of T. gondii infection during which IFN- KO and IL-12 KO
animals succumb. First, such acquired responses would be expected to
require longer to develop. Second, as already described, neither CTL
nor antibodies appear to contribute significantly to early immunity to
the parasite. Thus, elucidation of alternative mechanisms of
IFN--dependent protection is an important goal for the future.
ROLE OF T-CELL-MEDIATED IMMUNITY IN THE PATHOLOGIC CHANGES DUE TO TOXOPLASMOSIS
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Toxoplasma infection rapidly overcomes hosts with impaired T-cell function and diminished ability to produce type 1 cytokines. The parasite itself is a strong stimulus of this type of immunity, perhaps reflecting an advantage in keeping the host alive during infection. In recent years, it has become clear that type I and II cytokine responses must be tightly regulated for optimal control of infection and that cytokine imbalances resulting from loss of control can play a role in the pathologic changes associated with toxoplasmosis. Nevertheless, it is difficult to experimentally separate pathologic changes caused by the parasite through direct tissue destruction from more systemic damage caused by parasite-induced cytokines.
Acute Infection
The main pathologic findings associated with acute toxoplasmosis are lymphadenopathy and fever (57), which occur simultaneously with parasite-induced activation of the immune system and concurrent release of high levels of proinflammatory cytokines. In most patients, acute toxoplasmosis is benign and will evolve to the asymptomatic stage within a few weeks of infection.
Nevertheless, congenital transmission of Toxoplasma, occurring when a pregnant female is undergoing the acute phase of a primary infection, can result in severe disease in the infant. This important topic has been expertly reviewed by others (176). Briefly, the pathological consequences for the fetus are dependent upon the trimester during which transmission occurs, and will vary in severity from mild ocular disease to death (4, 176). In general, infection occurring during the early stages of pregnancy poses more of a health risk to the fetus than that occurring later, although the risk of transmission during maternal infection increases at later stages of pregnancy. The fetal pathologic findings are thought to be related to uncontrolled parasite replication in tissues and organs (4, 176). However, recent studies indicate that induction of a strong type 1 cytokine response at the fetal-maternal interface may also result in rejection of the fetus (233). Thus, such a response could contribute to spontaneous abortion during acute toxoplasmosis in a pregnant female.
The concept that T-cell-derived cytokines may promote pathologic
changes during Toxoplasma infection finds an experimental basis in recent studies on inflammatory mediator production during infection in mice. During oral infection, the susceptible C57BL/6 mouse
strain develops a severe gut inflammatory reaction, characterized by
severe necrosis of the villi and mucosal cells of the small intestine
(131). The pathologic changes are reversed by administration of either anti-IFN- or anti-CD4 MAb. Indeed, these two MAb also delay the onset of death in lethally infected animals (5,
131). While the function of IFN- in mediating this disease
process is not known, it has recently been demonstrated this cytokine induces Fas-dependent apoptosis of Peyer's patch T cells in orally infected mice (132). Additionally, depletion of another
inflammatory cytokine capable of triggering apoptosis, TNF
(239), delays the death of mice undergoing acute infection
with the highly virulent RH strain (144).
The above findings are reminiscent of the behavior of infected IL-10 KO animals. As described above, these mice, like orally infected C57BL/6 animals, appear to develop uncontrolled type 1 cytokine pathologic changes mediated by T lymphocytes (76, 163). In addition, recent studies in which D-galactosamine was used as a model to study mechanisms of low-dose endotoxicity suggest that granulocytes can contribute to the inflammatory pathologic changes triggered by Toxoplasma infection (144).
Thus, several lines of evidence suggest that Toxoplasma-induced inflammatory responses can contribute to the pathologic findings associated with acute infection in mice. T lymphocytes, in particular the CD4+ subset, as well as granulocytes, contribute to the response. In some circumstances, the parasite is capable of triggering a catastrophic inflammatory cytokine storm which the animals cannot survive. It is unclear whether similar pathologic changes are involved in clinical manifestations of human toxoplasmosis. Nevertheless, septic shock due to Toxoplasma has been described in patients infected with human immunodeficiency virus (HIV), suggesting that an uncontrolled inflammatory cytokine response may underlie this response (138).
Chronic Infection
In contrast to acute disease, most of the pathologic findings
associated with chronic toxoplasmosis in humans are thought to be
caused by lack of appropriate T-cell immunity rather than an
excessively vigorous response. Immunogenetic studies have demonstrated the influence of MHC loci on the development of TE, implicating T-cell
involvement in resistance (13, 16, 216, 217). Thus, the
Ld gene has been shown to confer resistance to
the development of disease (15), suggesting that
CD8+ responses restricted by this MHC class I molecule play
a protective role, through either IFN- production or CTL activity
(48). MHC class II-restricted CD4+ T lymphocytes
are similarly implicated by the finding that inbred mice carrying a
mutation in the class II A locus display increased cyst numbers
(16). The human MHC has also recently been linked to the
development of TE in AIDS patients (see below). Together, these genetic
studies reinforce the importance of MHC class I- and II-restricted
T-cell interactions in control of chronic disease.
Depletion of T cells in the setting of chronic infection rapidly leads
to reactivation of infection and death (61). The observation
that TE is accompanied by a large T-lymphocyte infiltrate may therefore
indicate that these cells are recruited to provide a protective
function with respect to disease (101, 193). A recent report
indicates that CD4+ cells in the infected brain appear to
produce several cytokines, including IL-2, IL-10, TNF-, IFN-, and
IL-4. The CD8+-T-cell subset produces a similar cytokine
spectrum, except that IL-4 is not produced but, instead, IL-1 is
synthesized (193). Thus, whether the protective effects of
CD4+ and CD8+ cells result from production of
proinflammatory or anti-inflammatory cytokines, or a balance of both,
is at present unclear. In contrast to findings suggesting that
CD4+ cells are required to prevent the reactivation of
chronic toxoplasmosis, it has been reported that CD4+
depletion can limit pathologic changes during this stage of disease (5, 107). These contradictory findings may possibly be
explained by the fact that inflammatory cytokines possess both
beneficial and harmful effects on the host and that the outcome of
infection is dependent upon tight regulation of these mediators.
Nevertheless, in general the fact remains that during T. gondii infection, TNF- and IFN- appear to be important for
control rather than exacerbation of chronic disease (Fig. 2) (61,
68, 213). Additionally, IL-6 KO mice display increased
susceptibility to TE as measured by increased cyst number and areas of
necrosis and tachyzoite-associated inflammation (219). The
KO animals also have smaller numbers of inflammatory mononuclear cell
infiltrates. Thus, IL-6 may mediate protection against TE by inducing
the accumulation of inflammatory cells, which act to limit infection.
This scenario provides a dramatic contrast to disease caused by another
apicomplexan parasite, Plasmodium falciparum. Thus, cerebral
malaria, which contributes to a large proportion of
Plasmodium-induced fatalities, results from the lethal
action of inflammatory cytokines and NO in the infected brain (79,
80).
Progression of chronic toxoplasmosis in mice correlates with rising
levels of IL-10 mRNA in the CNS (68). Since the latter cytokine modulates macrophage-mediated tachyzoite killing, production of anti-inflammatory mediators may contribute to TE susceptibility (74). An alternative, but not necessarily mutually
exclusive, view is that higher levels of IL-10 later during chronic
infection could play a role in down-regulating the inflammatory
response once the cyst burden has decreased and, in this way, could be beneficial for the host (18). In addition to IL-10, an early transient IL-4 response occurs in the brains of cyst-susceptible C57BL/10 mice (103). Furthermore, mice with a targeted
inactivation of the IL-4 gene, while more susceptible to acute oral
infection, display increased resistance to the establishment of tissue
cysts (179). The implication of the latter result is that
IL-4 acts to down-regulate CD4+-T cell-mediated type 1 cytokine-mediated pathologic changes during acute infection, as occurs
in the gut of susceptible mice after oral infection (131).
Nevertheless, in the chronically infected brain, IL-4 (like IL-10)
functions to down-regulate protective IFN-, thereby promoting
susceptibility to TE.
HIV-Related Pathology
In Toxoplasma-infected AIDS patients, or patients receiving immunosuppressive therapy, the parasite may reemerge, causing multiple CNS lesions with encephalopathy and focal neurological lesions (139, 162, 184). Up to 30% of HIV-positive patients infected with T. gondii will develop encephalitis caused by the parasite (146). Nevertheless, in recent years, the incidence of TE in the HIV-positive population has been decreasing, probably due to the use of the antibiotic trimethoprim-sulfamethoxazole as prophylaxis against both Pneumocystis carinii and T. gondii (17, 26, 209a).
Although an intense inflammatory process, characterized by the presence
of mononuclear and polymorphonuclear cells, is observed during TE in
HIV-positive patients (169), the major cause of CNS lesions
is most likely to be uncontrolled parasite replication itself rather
than immunopathologic changes. This hypothesis is supported by
experimental models involving neutralizing MAb to IFN- or TNF-,
or depletion of T lymphocytes (61, 68, 213). Further support
for T-cell involvement in preventing the development of TE comes from
studies indicating that expression of HLA-DQ3 confers susceptibility to
TE during AIDS progression (222). As revealed during HIV
infection, the enhanced susceptibility to T. gondii is
thought to be due mainly to decreased numbers of CD4+ T
lymphocytes, but it may also reflect a decrease in IL-12 synthesis or a
Th-cell switch from the Th1 to Th0 phenotype (63, 142).
Because several proinflammatory monokines are able to enhance HIV-1
replication in vitro (51, 126, 172), we decided to investigate the effects of T. gondii infection on HIV-1
replication in vitro and in vivo. As might be predicted, in vitro
stimulation with tachyzoite products enhances viral replication in
primary human macrophages (7). Similarly,
Toxoplasma infection of transgenic mice containing multiple
copies of HIV-1 proviral DNA triggers the expression of HIV-1-specific
mRNA through induction of NF-B (60). These findings
suggest a double-barreled impact of HIV infection on chronic
Toxoplasma infection. Thus, T. gondii is capable
of enhancing HIV-1 replication within reservoir host cells; simultaneously, HIV-1 itself undermines acquired immunity to the parasite, promoting reactivation of chronic toxoplasmosis.
Ocular Toxoplasmosis
Congenital ocular toxoplasmosis is another disease manifestation
which occurs late during the chronic stage of infection
(176). In most instances, ocular lesions are thought to be
caused by tissue damage due to parasite proliferation. Indeed, studies
in the mouse model show that in vivo neutralization of TNF- or
IFN-, or in vivo treatment with iNOS inhibitors, also results in an intense uveitis that is associated with tachyzoite emergence and increased cyst number (64, 87).
While most cases of ocular toxoplasmosis originate from congenital
transmission, this disease manifestation can occur during normally
acquired infection. In this regard, epidemiological studies performed
on a patient population in Erexim (situated in southern Brazil)
indicate that the frequency of acquired ocular toxoplasmosis at this
location is extremely high (77). Comparative studies on
patients with acquired and congenital ocular disease indicate that the
latter group displays very weak delayed-type hypersensitivity, as well
as low T-cell proliferation and Th1 cytokine production in response to
T. gondii (178). Indeed, other studies have also found that congenitally infected patients in general possess lower -T-cell responsiveness later in life (84, 148).
These results are consistent with the hypothesis that congenital ocular disease may be a consequence of immunological tolerance developed by the infected fetus, manifesting later in life as an inability to control parasite growth. The data also raise the possibility that acquired ocular disease occurs as a result of an excessive inflammatory reaction elicited by parasite Ag in the eye tissues. Nevertheless, during this and other forms of Toxoplasma-associated pathologic changes (with only a few exceptions [76, 131, 144]), it is difficult to establish a detrimental role for inflammatory cytokines, since these mediators must be present to prevent lethal parasitemia.
CONCLUSIONS AND FUTURE DIRECTIONS
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A wealth of data point to the major role of T lymphocytes and type
1 cytokines during experimental T. gondii infection. In the
clinical setting, this is dramatically confirmed by the emergence of
Toxoplasma as a major opportunistic infection during the
progression of AIDS. Much progress has been made in recent years in
determining how early innate responses, namely, production of cytokines
such as IL-12 and TNF-, drive the development of the strong Th1
response to Toxoplasma. While this response is crucial to
the control of infection, it is becoming increasingly appreciated that
an excessively vigorous response induced by the parasite can lead to
pathologic changes in the host. Therefore, it is important for the host
and parasite to maintain tight control of the inflammatory response. Within the teleological context of the host-parasite relationship, Toxoplasma may seek to induce strong protective host
immunity, because without such a response, the parasite rapidly
overcomes the host, resulting in host death and consequently in a
minimal chance for the parasite to transmit itself to a new host.
Recent studies have revealed several distinct clonal Toxoplasma lineages, defined by genetic polymorphism, which differ in virulence and epidemiological patterns of occurrence (205). Variation in the spectrum of Ag expressed by the parasite is also known to occur among different Toxoplasma isolates (231). Moreover, it is probable that strain- or isolate-specific variation exerts an effect on host control of infection (215). Defining the immunological basis of such differences at the cellular and molecular level will probably be an important issue in the future.
Several distinct cell types are important in triggering and sustaining
the type 1 cytokine response (e.g., CD4+ and
CD8+ T lymphocytes, T cells, and non-T cells such as
macrophages, dendritic cells, and neutrophils), and it is therefore
probable that distinct parasite Ag are involved in activation of the
different cell types. Identification and cloning of these parasite
molecules will be an important effort in the next several years,
hopefully leading to development of vaccines capable of preventing the
serious manifestations of toxoplasmosis, and, in general, deepening our knowledge of how opportunistic pathogens interact with their hosts.
ACKNOWLEDGMENTS
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We thank G. Yap for a critical review of the manuscript.
R.T.G. is supported by a Biotechnology Career Fellowship from the Rockefeller Foundation and a grant from the Brazilian Government (CNPq 522.056/95-4). E.Y.D. is supported by the USDA Hatch Act and Animal Health and Disease Research Program (Cornell University College of Veterinary Medicine) and the National Institutes of Health (AI-40540).
FOOTNOTES
* Corresponding author. Mailing address: Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853-6401. Phone: (607) 253-4022. Fax: (607) 253-3384. E-mail: eyd1{at}cornell.edu.
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Masek, K. S., Fiore, J., Leitges, M., Yan, S.-F., Freedman, B. D., Hunter, C. A.
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Wilson, E. H., Zaph, C., Mohrs, M., Welcher, A., Siu, J., Artis, D., Hunter, C. A.
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177: 2365-2372
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Kim, L., Butcher, B. A., Lee, C. W., Uematsu, S., Akira, S., Denkers, E. Y.
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Gilbert, R, Dezateux, C
(2006). Newborn screening for congenital toxoplasmosis: feasible, but benefits are not established.. Arch. Dis. Child.
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Bennouna, S., Sukhumavasi, W., Denkers, E. Y.
(2006). Toxoplasma gondii Inhibits Toll-Like Receptor 4 Ligand-Induced Mobilization of Intracellular Tumor Necrosis Factor Alpha to the Surface of Mouse Peritoneal Neutrophils. Infect. Immun.
74: 4274-4281
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Kim, L., Denkers, E. Y.
(2006). Toxoplasma gondii triggers Gi-dependent PI 3-kinase signaling required for inhibition of host cell apoptosis. J. Cell Sci.
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Subauste, C. S., Wessendarp, M.
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74: 1573-1579
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Mullarky, I. K., Szaba, F. M., Berggren, K. N., Kummer, L. W., Wilhelm, L. B., Parent, M. A., Johnson, L. L., Smiley, S. T.
(2006). Tumor Necrosis Factor Alpha and Gamma Interferon, but Not Hemorrhage or Pathogen Burden, Dictate Levels of Protective Fibrin Deposition during Infection. Infect. Immun.
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Yazar, S., Gur, M., Ozdogru, I., Yaman, O., Oguzhan, A., Sahin, I.
(2006). Anti-Toxoplasma gondii antibodies in patients with chronic heart failure. J Med Microbiol
55: 89-92
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Rozenfeld, C., Martinez, R., Seabra, S., Sant'Anna, C., Goncalves, J. G. R., Bozza, M., Moura-Neto, V., De Souza, W.
(2005). Toxoplasma gondii Prevents Neuron Degeneration by Interferon-{gamma}-Activated Microglia in a Mechanism Involving Inhibition of Inducible Nitric Oxide Synthase and Transforming Growth Factor-{beta}1 Production by Infected Microglia. Am. J. Pathol.
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Villarino, A. V., Larkin, J. III, Saris, C. J. M., Caton, A. J., Lucas, S., Wong, T., de Sauvage, F. J., Hunter, C. A.
(2005). Positive and Negative Regulation of the IL-27 Receptor during Lymphoid Cell Activation. J. Immunol.
174: 7684-7691
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Mayer, K. D., Mohrs, K., Crowe, S. R., Johnson, L. L., Rhyne, P., Woodland, D. L., Mohrs, M.
(2005). The Functional Heterogeneity of Type 1 Effector T Cells in Response to Infection Is Related to the Potential for IFN-{gamma} Production. J. Immunol.
174: 7732-7739
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Smiley, S. T., Lanthier, P. A., Couper, K. N., Szaba, F. M., Boyson, J. E., Chen, W., Johnson, L. L.
(2005). Exacerbated Susceptibility to Infection-Stimulated Immunopathology in CD1d-Deficient Mice. J. Immunol.
174: 7904-7911
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Kim, L., Rio, L. D., Butcher, B. A., Mogensen, T. H., Paludan, S. R., Flavell, R. A., Denkers, E. Y.
(2005). p38 MAPK Autophosphorylation Drives Macrophage IL-12 Production during Intracellular Infection. J. Immunol.
174: 4178-4184
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Staska, L. M., Davies, C. J., Brown, W. C., McGuire, T. C., Suarez, C. E., Park, J. Y., Mathison, B. A., Abbott, J. R., Baszler, T. V.
(2005). Identification of Vaccine Candidate Peptides in the NcSRS2 Surface Protein of Neospora caninum by Using CD4+ Cytotoxic T Lymphocytes and Gamma Interferon-Secreting T Lymphocytes of Infected Holstein Cattle. Infect. Immun.
73: 1321-1329
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Gubbels, M.-J., Striepen, B., Shastri, N., Turkoz, M., Robey, E. A.
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73: 703-711
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Pepper, M., Dzierszinski, F., Crawford, A., Hunter, C. A., Roos, D.
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72: 7240-7246
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Mason, N. J., Fiore, J., Kobayashi, T., Masek, K. S., Choi, Y., Hunter, C. A.
(2004). TRAF6-Dependent Mitogen-Activated Protein Kinase Activation Differentially Regulates the Production of Interleukin-12 by Macrophages in Response to Toxoplasma gondii. Infect. Immun.
72: 5662-5667
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Collette, A., Bagot, S., Ferrandiz, M. E., Cazenave, P.-A., Six, A., Pied, S.
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Liesenfeld, O., Dunay, I. R., Erb, K. J.
(2004). Infection with Toxoplasma gondii Reduces Established and Developing Th2 Responses Induced by Nippostrongylus brasiliensis Infection. Infect. Immun.
72: 3812-3822
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Portugal, L. R., Fernandes, L. R., Cesar, G. C., Santiago, H. C., Oliveira, D. R., Silva, N. M., Silva, A. A., Lannes-Vieira, J., Arantes, R. M. E., Gazzinelli, R. T., Alvarez-Leite, J. I.
(2004). Infection with Toxoplasma gondii Increases Atherosclerotic Lesion in ApoE-Deficient Mice. Infect. Immun.
72: 3571-3576
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Del Rio, L., Butcher, B. A., Bennouna, S., Hieny, S., Sher, A., Denkers, E. Y.
(2004). Toxoplasma gondii Triggers Myeloid Differentiation Factor 88-Dependent IL-12 and Chemokine Ligand 2 (Monocyte Chemoattractant Protein 1) Responses Using Distinct Parasite Molecules and Host Receptors. J. Immunol.
172: 6954-6960
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Hancock, W. W., Szaba, F. M., Berggren, K. N., Parent, M. A., Mullarky, I. K., Pearl, J., Cooper, A. M., Ely, K. H., Woodland, D. L., Kim, I.-J., Blackman, M. A., Johnson, L. L., Smiley, S. T.
(2004). Intact type 1 immunity and immune-associated coagulative responses in mice lacking IFN{gamma}-inducible fibrinogen-like protein 2. Proc. Natl. Acad. Sci. USA
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Gavrilescu, L. C., Butcher, B. A., Del Rio, L., Taylor, G. A., Denkers, E. Y.
(2004). STAT1 Is Essential for Antimicrobial Effector Function but Dispensable for Gamma Interferon Production during Toxoplasma gondii Infection. Infect. Immun.
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Kim, L., Butcher, B. A., Denkers, E. Y.
(2004). Toxoplasma gondii Interferes with Lipopolysaccharide-Induced Mitogen-Activated Protein Kinase Activation by Mechanisms Distinct from Endotoxin Tolerance. J. Immunol.
172: 3003-3010
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Bennouna, S., Bliss, S. K., Curiel, T. J., Denkers, E. Y.
(2003). Cross-Talk in the Innate Immune System: Neutrophils Instruct Recruitment and Activation of Dendritic Cells during Microbial Infection. J. Immunol.
171: 6052-6058
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Ismael, A. B., Sekkai, D., Collin, C., Bout, D., Mevelec, M.-N.
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71: 6222-6228
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Fux, B., Rodrigues, C. V., Portela, R. W., Silva, N. M., Su, C., Sibley, D., Vitor, R. W. A., Gazzinelli, R. T.
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71: 6392-6401
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Fachado, A., Rodriguez, A., Molina, J., Silverio, J. C., Marino, A. P. M. P., Pinto, L. M. O., Angel, S. O., Infante, J. F., Traub-Cseko, Y., Amendoeira, R. R., Lannes-Vieira, J.
(2003). Long-Term Protective Immune Response Elicited by Vaccination with an Expression Genomic Library of Toxoplasma gondii. Infect. Immun.
71: 5407-5411
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Staska, L. M., McGuire, T. C., Davies, C. J., Lewin, H. A., Baszler, T. V.
(2003). Neospora caninum-Infected Cattle Develop Parasite-Specific CD4+ Cytotoxic T Lymphocytes. Infect. Immun.
71: 3272-3279
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Johnson, L. L., Berggren, K. N., Szaba, F. M., Chen, W., Smiley, S. T.
(2003). Fibrin-mediated Protection Against Infection-stimulated Immunopathology. JEM
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Tato, C. M., Villarino, A., Caamano, J. H., Boothby, M., Hunter, C. A.
(2003). Inhibition of NF-{kappa}B Activity in T and NK Cells Results in Defective Effector Cell Expansion and Production of IFN-{gamma} Required for Resistance to Toxoplasma gondii. J. Immunol.
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Straw, A. D., MacDonald, A. S., Denkers, E. Y., Pearce, E. J.
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Scorza, T., D'Souza, S., Laloup, M., Dewit, J., De Braekeleer, J., Verschueren, H., Vercammen, M., Huygen, K., Jongert, E.
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King, M. D., Lindsay, D. S., Holladay, S., Ehrich, M.
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(2002). The Function of Gamma Interferon-Inducible GTP-Binding Protein IGTP in Host Resistance to Toxoplasma gondii Is Stat1 Dependent and Requires Expression in Both Hematopoietic and Nonhematopoietic Cellular Compartments. Infect. Immun.
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Butcher, B. A., Denkers, E. Y.
(2002). Mechanism of Entry Determines the Ability of Toxoplasma gondii To Inhibit Macrophage Proinflammatory Cytokine Production. Infect. Immun.
70: 5216-5224
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Silva, N. M., Rodrigues, C. V., Santoro, M. M., Reis, L. F. L., Alvarez-Leite, J. I., Gazzinelli, R. T.
(2002). Expression of Indoleamine 2,3-Dioxygenase, Tryptophan Degradation, and Kynurenine Formation during In Vivo Infection with Toxoplasma gondii: Induction by Endogenous Gamma Interferon and Requirement of Interferon Regulatory Factor 1. Infect. Immun.
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Feron, E. J., Klaren, V. N. A., Wierenga, E. A., Verjans, G. M. G. M., Kijlstra, A.
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Butcher, B. A., Kim, L., Johnson, P. F., Denkers, E. Y.
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Bliss, S. K., Gavrilescu, L. C., Alcaraz, A., Denkers, E. Y.
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69: 4898-4905
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Gavrilescu, L. C., Denkers, E. Y.
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167: 902-909
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Schöler, N., Krause, K., Kayser, O., Müller, R. H., Borner, K., Hahn, H., Liesenfeld, O.
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Caamano, J., Tato, C., Cai, G., Villegas, E. N., Speirs, K., Craig, L., Alexander, J., Hunter, C. A.
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Tarleton, R. L., Grusby, M. J., Zhang, L.
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Reichmann, G., Walker, W., Villegas, E. N., Craig, L., Cai, G., Alexander, J., Hunter, C. A.
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Vercammen, M., Scorza, T., Huygen, K., De Braekeleer, J., Diet, R., Jacobs, D., Saman, E., Verschueren, H.
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Bliss, S. K., Zhang, Y., Denkers, E. Y.
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Doherty, T. M., Chougnet, C., Schito, M., Patterson, B. K., Fox, C., Shearer, G. M., Englund, G., Sher, A.
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Bliss, S. K., Marshall, A. J., Zhang, Y., Denkers, E. Y.
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