TABLE 5

PCR-based assays for detection and molecular characterization of Cyclospora f

SpeciesAmplification methodDetection methodTargetSample type(s)Reference
Cyclospora, Eimeria Nested PCRGel electrophoresisSSU rDNAPurified oocysts 464
Cyclospora, Eimeria Nested PCR a Gel electrophoresisSSU rDNAStool 465
Cyclospora, Eimeria Nested PCR-RFLP a Gel electrophoresisSSU rDNARaspberries 466
Cyclospora, Eimeria Nested PCR a Oligonucleotide ligationSSU rDNAControls 467
Cyclospora, Eimeria Nested PCR a SequencingSSU rDNAStool 468
Cyclospora PCRGel electrophoresisITS1 188
Cyclospora Real-time PCRHydrolysis probeSSU rDNAControls 190
Cyclospora Real-time PCRHydrolysis probeSSU rDNAStool 191
Cyclospora, Eimeria Nested PCR-RFLPGel electrophoresisSSU rDNAWater 469
C. cayetanensis, C. colobi, and Eimeria Multiplex nested PCR b Gel electrophoresisSSU rDNAStool 470
Cyclospora, Eimeria Nested PCR a Gel electrophoresisSSU rDNASputum 471
Cyclospora, Eimeria Nested PCR a Gel electrophoresisSSU rDNAStool 472
Cyclospora Real-time PCR e Hydrolysis probeSSU rDNAStool 473
C. cayetanensis, C. colobi, and Eimeria Multiplex Nested PCR d Gel electrophoresisSSU rDNAStool 194
Cyclospora, Eimeria Nested PCR-RFLP a Gel electrophoresisSSU rDNAStool 192
C. cayetanensis PCRGel electrophoresisITS2Spiked samples 474
Cyclospora PCR c Gel electrophoresisITS1Stool 189
Nested PCRGel electrophoresisSSU rDNAControls 450
Cyclospora, Cryptosporidium, Toxoplasma, and Sarcocystis Real-time PCRSYBR green MCASSU rDNAControl samples 219
C. cayetanensis, C. colobi, and Eimeria Multiplex PCR d LuminexSSU rDNAStool 193
C. cayetanensis, C. colobi, and Eimeria Multiplex nested PCR d Gel electrophoresisSSU rDNAStool 184
Cyclospora Nested PCRSequencingSSU rDNAStool 475
Cyclospora Nested PCRSequencingHSP70Stool 195
C. cayetanensis, C. colobi, and Eimeria Nested PCR d ElectrophoresisSSU rDNARaspberry, basil, pesto 476
C. cayetanensis, C. cercopitheci Real-time PCRHydrolysis probeSSU rDNARaspberry, basil, pesto
C. cayetanensis Real-time PCRHydrolysis probeHSP70Raspberry, basil, pesto
  • a PCR primers are described in reference 464.

  • b First-round PCR primers are described in reference 464.

  • c PCR primers are described in reference 188.

  • d PCR primers are described in reference 470.

  • e PCR primers are described in reference 190.

  • f Abbreviations: SSU, small subunit; ITS, internal transcribed spacer; HSP, heat shock protein; RFLP, restriction fragment length polymorphism; MCA, melt curve analysis.