TABLE 10

PCR-based assays for detection and molecular characterization of cestode infections g

Genus or speciesAmplification methodDetection methodTarget(s)Sample type(s) (no. of samples)Reference(s)
Diphyllobothrium genusMultiplex PCRGel electrophoresis cox1 Proglottids, eggs 407
T. saginata PCRGel electrophoresisHDP1Proglottids 528, 529
T. solium, T. saginata Multiplex PCRGel electrophoresisHDP2Proglottids
T. solium, T. saginata Multiplex PCR-RFLP e Gel electrophoresisHDP2Proglottids 530
T. solium, T. saginata, T. asiatica PCR-RFLPGel electrophoresisMitochondrial 12S rDNAProglottids 531
T. solium, T. saginata Multiplex PCR e Gel electrophoresisHDP2Stool 532
T. solium, T. saginata, T. asiatica Multiplex PCRGel electrophoresis cox1 Proglottids, stool (6) 533
T. saginata, T. asiatica Multiplex PCR-RFLPGel electrophoresisHDP2Proglottids 534
T. solium, T. saginata, T. asiatica Multiplex PCR a Gel electrophoresis cox1 Proglottids, stool (25) 535
T. solium, T. saginata PCR-RFLPGel electrophoresis cox1 Stool (12) 536
T. solium PCRGel electrophoresisTsol9 repeat sequenceCerebrospinal fluid 404
T. solium, T. saginata, T. asiatica PCR-RFLP b Gel electrophoresisMitochondrial 12S rDNAProglottids 537, 538
T. solium, T. saginata, T. asiatica Multiplex PCR a Gel electrophoresis cox1 Proglottids, stool (19) 539
T. solium, T. saginata, T. asiatica Multiplex PCR a Gel electrophoresis cox1 Proglottids 540
T. solium, T. saginata, T. asiatica Multiplex PCR a Gel electrophoresis cox1 Proglottids 541
T. solium Nested PCRGel electrophoresisTsol31Stool (155) 542
T. solium Nested PCRGel electrophoresisHDP2Cerebrospinal fluid 543
Taenia genusPCRSequencingITS1Cerebrospinal fluid 425
T. solium, T. saginata, T. asiatica Multiplex PCRGel electrophoresis cox1 Proglottids 544
T. solium, T. saginata, T. asiatica LAMPGel electrophoresisClp, cox1 Proglottids, cysticerci, stool (6) 545
T. solium, T. saginata, T. asiatica LAMP f Gel electrophoresisClp, cox1 Stool (43) 403
T. solium, T. saginata, T. asiatica PCRGel electrophoresisHDP2Proglottids 546
T. solium, T. saginata, T. asiatica Multiplex PCR a Gel electrophoresis cox1 Proglottids 547
T. solium PCR d Gel electrophoresisTsol9 repeat sequenceCerebrospinal fluid 405
T. solium Real-time PCRLNA hydrolysis probeTsol9 repeat sequenceCerebrospinal fluid 406
T. solium, T. saginata, T. asiatica Multiplex PCR c Gel electrophoresis cox1 Proglottids, eggs 548
T. solium, T. saginata, T. asiatica PCR-RFLP b Gel electrophoresisMitochondrial 12S rDNAProglottids 537
T. solium, T. saginata, T. asiatica PCR-RFLP b Gel electrophoresisMitochondrial 12S rDNAProglottids 549
T. solium, T. saginata, T. asiatica Multiplex PCR c Gel electrophoresis cox1 Proglottids 550
T. solium, T. saginata Real-time multiplex PCRHydrolysis probeITS1Stool (817) 402
  • a PCR primers are described in reference 533.

  • b PCR primers are described in reference 531.

  • c PCR primers are described in reference 544.

  • d PCR primers are described in reference 404.

  • e PCR primers are described in references 528 and 529.

  • f LAMP primers were described previously (545).

  • g Abbreviations: RFLP, restriction fragment length polymorphism; LNA, locked nucleic acid; SSU, small subunit; ITS, internal transcribed spacer; cox1, mitochondrial cytochrome c oxidase subunit I gene; Clp, cathepsin L-like cysteine peptidase; HDP1, T. saginata-specific repetitive sequence; HDP2, cestode-specific sequence.