TABLE 1

Performance of culture methods and nucleic acid amplification assays for detecting Kingella kingae in children with skeletal system infectionsa

YrReferenceCulture methodNo. with positive K. kingae culture/totalPCR methodTarget gene No. with positive PCR/total
C-PCRRT-PCR
1998 301 C0/1C16S rRNA geneNA1/1
2003 186 C + BCV13/18C16S rRNA geneNA18/18
2005 303 C + BCV9/24C16S rRNA geneNA24/24
2007 302 C + BCV6/21C + RT16S rRNA gene16S rRNA gene20/21
2007 16 C + BCV17/39C + RT16S rRNA gene cpn60 39/39
2008 200 BCV2/4C + RT16S rRNA gene cpn60 4/4
2008 206 C0/1C16S rRNA geneNA1/1
2009 15 C + BCV7/31RTND cpn60 31/31
2009 88 C0/2C + RT16S rRNA gene rtxA + rtxB 2/2
2009 158 C + BCV5/18RTND?18/18
2010 89 C0/23RTND rtxA + rtxB 23/23
2011 90 ND2/20RTND rtxA 20/20
2013 151 C + BCV5/44RTND cpn60 43/44
2013 152 C?C + RT16S rRNA gene cpn60 11/11
2014 160 C + BCV2/24C + RTND rtxA 27/27
2014 304 C0/3RTND cpn60 3/3
2014 305 C + BCV0/1RTND cpn60 1/1
Total 68/274 (24.8%) 388/390 (99.5%)
  • a Abbreviations: C, conventional; BCV, blood culture vial; RT, real time; ND, not done; NA, not applicable.