TABLE 4

Conjugative plasmids encoding RND or ABC multicomponent efflux systems of Gram-negative bacteria

Plasmid(s) (GenBank accession no.)Isolation yrSource, countryHostInc groupSize (kb)Efflux component(s)Pump regulatorAdditional resistance gene(s) and transposon(s)Resistance phenotype of initial host strainReferences
pMG101 (AF067954)1973Burn ward, USA Salmonella enterica serovar TyphimuriumIncHI180SilABC RND and SilP ABC transportersSilRSUnknownAmpicillin, chloramphenicol, streptomycin, tetracycline, silver 716, 717
pMCBF1 (AY950444), pMCBF6 (EF107516)1993Marine, SwedenUncultured bacterial biofilm (Pseudomonas putida as recipient)IncP-163, 67MexEF-OprN RND homolog and MerF/MerT transportersMerR merA, merB, merD, merP; Tn5053 or Tn5058 Mercury 721, 722
pOLA52 (EU370913)1999Swine manure, Denmark Escherichia coli IncX152OqxAB RND pumpORF68 (GntR family) bla TEM; Tn6011 Ampicillin, carbadox, chloramphenicol, kanamycin, nitrofurantoin, olaquindox, sulfamethoxazole, trimethoprim 708, 892, 1018, 1019
pB4 (AJ431260)1999Activated sludge, GermanyUncultured bacterium (Pseudomonas knackmussii as recipient)IncP-1β79MexCD-OprJ RND homologNfxB bla NPS-1, chrABC, strA, strB; Tn5719, Tn5720, Tn5393c β-Lactams, spectinomycin, streptomycin, tetracycline, chromate 1020, 1021
pRSB101 (AJ698325)2001Activated sludge, GermanyUncultured bacterium (Pseudomonas knackmussii as recipient)IncQ48AcrA-ABC permease/ATPase, OprM and TetA MSF pumpAcrR and TetR aadA2, bla TLA-2, chrA, dhfr1, mph(A), mphR(A); qacEΔ, sulI, Tn402, and intI Cephalosporins, erythromycin, nalidixic acid, norfloxacin, roxithromycin, spectinomycin, streptomycin, sulfonamides, tetracycline, trimethoprim 719, 1021
pNDM-CIT (JX182975)2010Patient, India/France Citrobacter freundii IncHI1289MexAB-CusC RND homologAcrR, HN-S-like protein bla NDM-1, armA, mel, mph; Tn1548 β-Lactams, aminoglycoside, fluoroquinolones, macrolides, nitrofurantoin, tetracycline, tigecycline 243, 724