TABLE 7.

Kinetic properties of representative class A inhibitor-resistant and wild-type enzymesa

β-LactamaseClavulanateSulbactamTazobactamReference(s)
KI (μM)kinact (s−1)tnKI (μM)kinact (s−1)tnKI (μM)kinact (s−1)tn
TEM-10.10.021601.60.00210,0000.01>0.02140 73, 180
TEM Met69Leu1.650.0022,800220.00097,0000.200.0042,900 73
TEM Ser130Gly1050.0031,6002200.031,900950.04630 408
TEM Arg244Ser33440.000017,800 179, 180
TEM Asn276Asp15.60.01250 378
TEM Met69Leu, Asn276Asp27490.6 386
SHV-110.04608.60.05613,0000.070.15 153, 410, 411
SHV Arg244Ser630.09502400.13100 410, 411
SHV Ser130Gly470.03404.20.065 153
OHIO-10.40.0180170.00140,0000.300.01400 229
OHIO Met69Ile100.0022,000680.0004200,000180.00162,000 229
  • a The definition of KI may not be uniform from laboratory to laboratory. See the cited references for the methods used in each determination.