Comparison of commercial molecular assays for diagnosis of sepsis reviewed in this paper

AssayManufacturerSalient feature(s)Detectable pathogensaDetection limit (CFU/ml)bTurnaround time (h)Reference(s)
Performed on positive blood culture bottles
    PNA-FISHAdvanDX, Woburn, MAFluorescence-based hybridization with PNA probesDifferent pathogen-specific kits availableNA358, 59, 76, 77, 113, 150, 183, 213
    Hyplex BloodScreenBAG, Lich, GermanyMultiplex PCR with subsequent hybridization on an ELISA plate10 different pathogens and mecA geneNA3208
    Prove-it SepsisMobidiag, Helsinki, FinlandMultiplex PCR with subsequent hybridization on a microarray50 different pathogens and mecA geneNA3None
Performed directly on whole blood
    SepsiTestMolzym, Bremen, GermanyBroad-range PCR with subsequent sequencing>300 different pathogens20-40 for S. aureus8-12132
    VyooSIRS-Lab, Jena, GermanyMultiplex PCR with subsequent gel electrophoresis>40 different pathogens and mecA, vanA, vanB, vanC, and blaSHV genes3-108None
    LightCycler SeptiFast TestRoche Molecular Systems, Branchburg, NJMultiplex real-time PCR25 different pathogens3-30622, 101, 112, 115, 116, 134, 148, 185, 198, 200, 201, 210
  • a See the text for details.

  • b NA, not available.