TABLE 2.

Physical and kinetic parameters

Enzyme classSourceLocationaMolecular mass (kDa)pIRelative kcatReference(s)
BenzylpenicillinAmpicillinCefazolinCephaloridineCefoxitinCefotaximeImipenemc
CE. cloacaeChr39.28.4100521,4005,0000.430.110.0299, 100, 211
C. freundiiChr39.91002116,1002,26010.050.0599, 100, 287
E. coli K-12Chr39.68.710093332890.440.380.0299, 100, 144, 212
S. marcescensChr379.51000.61,7301,4700.022.30.00199, 100, 148
P. aeruginosaChr348.41006b1450.0150.200.0399, 100, 102, 221
ACT-1P39.49.01001.81,020>4550.670.090.0225, 41
MIR-1P39.28.41003.9b1,5404.619.30.0925, 248
CMY-1P39.98.01003.52,5001,1900.380.080.0220, 25
CMY-2P38.89.01003.9b1,5361.640.290.2422, 25
ATEM-2P22.05.41009534315204
B. licheniformisChr29.5100681429204
DOXA-29Chr28.5>9100164170.26NH96
  • a Chr, chromosomal; P, plasmid.

  • b Biphasic kinetics.

  • c NH, no hydrolysis detected.