TABLE 2.

Physical and kinetic parameters

Enzyme classSourceLocationaMolecular mass (kDa)pIRelative kcatReference(s)
BenzylpenicillinAmpicillinCefazolinCephaloridineCefoxitinCefotaximeImipenemc
C E. cloacae Chr39.28.4100521,4005,0000.430.110.02 99, 100, 211
C. freundii Chr39.91002116,1002,26010.050.05 99, 100, 287
E. coli K-12Chr39.68.710093332890.440.380.02 99, 100, 144, 212
S. marcescens Chr379.51000.61,7301,4700.022.30.001 99, 100, 148
P. aeruginosa Chr348.41006b1450.0150.200.03 99, 100, 102, 221
ACT-1P39.49.01001.81,020>4550.670.090.02 25, 41
MIR-1P39.28.41003.9b1,5404.619.30.09 25, 248
CMY-1P39.98.01003.52,5001,1900.380.080.02 20, 25
CMY-2P38.89.01003.9b1,5361.640.290.24 22, 25
ATEM-2P22.05.41009534315 204
B. licheniformis Chr29.5100681429 204
DOXA-29Chr28.5>9100164170.26NH 96
  • a Chr, chromosomal; P, plasmid.

  • b Biphasic kinetics.

  • c NH, no hydrolysis detected.