TABLE 4.

In vitro drug susceptibilities of Scedosporium spp.

Species and drugReference(s)No. of strainsMethodaEndpointsbIncubation time (h)MIC, μg/ml
RangeMedian geometric mean (range)50% (range)90% (range)
S. prolificans
    Miconazole 86, 28698M38-A-likeMIC-0, -148-724->649.85->168->16>16-64
    Itraconazole 68, 86, 286131M38-A-likeMIC-0, -1, -248-728->3215.36 (16->32)>16 (>16->32)>16 (>16->32)
117, 4305EtestNo growth48>32NAcNANA
347 6Agar dilutionNo growthNA>64NANANA
69 18Yeast oneRed well72-120≥16NANA≥16
    Albaconazole 68, 28698M38-AMIC-1, -2720.01-80.35-1.970.5-22-4
    Voriconazole 68, 86, 286131M38-A-likeMIC-0, -1, -248-720.06-323.29 (1.83-16)4 (2-16)4 (4-16)
347 6Agar dilutionNo growthNA4NANANA
    Posaconazole 68, 28688M38-AMIC-1, -2720.25->16>8-10.55>8-16>8-16
    Ravuconazole 68, 8568M38-A-likeMIC-0, -248-720.5->168.9->8>8-16>8-16
    Fluconazole 86 43M38-A-likeMIC-048>16>16>16>16
69 18Yeast oneRed well72-12032-≥256NANA≥256
    Ketoconazole 68, 8678M38-A-likeMIC-0, -248-720.5->1611.52->16>16>16
69 18Yeast oneRed well72-120≥16NANA≥16
    Terbinafine 141, 28675M38-A-likeMIC-0, -148-722->3216.5-19.161616-32
    Amphotericin B 68, 86, 286131M38-A-likeMIC-048-720.125->16>16 (6.48->16)>16 (8->16)>16 (16->16)
117, 4305EtestNo growth48>32NANANA
69 18Yeast oneRed well72-1202->16NANA>16
    Nystatin 68, 28688M38-AMIC-0724->324.13-32>16-32>16->32
    l-Nystatin 286 55M38-AMIC-07216-32323232
    Flucytosine 86 43M38-A-likeMIC-048>16>16>16>16
69 18Yeast oneRed well72-120≥64NANA≥64
    Caspofungin 116 2M38-AMIC-2724-8NANANA
99 2Broth macrodilution80%726.25-12.58.83NANA
    Anidulafungin 116, 3217M38-AMIC-2724->168NANA
    Micafungin 489 17M38-AMIC-248>32>32>32>32
188 3XTT colorMIC-224>128NA>128NA
S. apiospermum
    Miconazole 86, 286, 49257M38-A-likeMIC-0, -148-720.06-40.5-0.580.5 (0.25-0.5)1 (0.5-2)
    Itraconazole 68, 86, 162, 286, 49296M38-A-likeMIC-0, -1, -248-720.03->161.56 (0.78-8)2 (0.5->16)4 (1->16)
117, 4305EtestNo growth480.5-32NA1.5>32
347 6Agar dilutionNo growthNA0.12->64NANANA
69 10Yeast oneRed well72-1200.5-≥16NANA≥16
    Albaconazole 68, 28624M38-A-likeMIC-1,-2720.06-20.41-10.5-11-2
    Voriconazole 68, 86, 162, 286, 49296M38-A-likeMIC-0, -1, -248-720.01-20.17 (0.06-1.13)0.25 (0.06-2)0.5 (0.125-2)
347 6Agar dilutionNo growthNA0.12-0.5NANANA
251 6Yeast onePurple well721NA0.51
    Posaconazole 68, 162, 28652M38-A-likeMIC-1, -2720.03-20.79 (0.08-1.05)1 (0.03-1)2 (0.25-2)
    Ravuconazole 68, 162, 28693M38-A-likeMIC-0, -248-720.125-82.21 (0.125-3.5)2 (0.125-4)4 (0.125->8)
    Fluconazole 86, 162, 49272M38-A-likeMIC-0, -1, -248-724-6416 (12.16->16)16 (16->16)32 (>16->64)
69 10Yeast oneRed well72-12016-≥256NANA≥256
    Ketoconazole 68, 8638M38-A-likeMIC-0, -248-720.06->162-10.072-16<16
69 10Yeast oneRed well72-1200.25-≥16NANA≥16
    Terbinafine 141, 28644M38-A-likeMIC-0, -148-728->3217.1->32>32>32->16
    Amphotericin B 86, 286, 49268M38-A-likeMIC-048-721->164 (2.97-4)4 (4-4)16 (16->16)
117, 4305EtestNo growth482->32NA>32>32
69 10Yeast oneRed well72-1202->16NANA4
    Nystatin 68, 28624M38-AMIC-0724-3212.7-13.2416>16-32
    l-Nystatin 286 13M38-AMIC-0724-1611.991616
    Flucytosine 85, 49244M38-A-likeMIC-0, -148-728->64>16->64>16->64>16
69 10Yeast oneRed well72-1204-≥64NANA≥64
    Caspofungin 116, 33411M38-AMIC-1, -2720.25-41.30.5NA
99 4Broth macrodilution80%720.19-0.780.38NANA
    Anidulafungin 116, 321, 33417M38-AMIC-1, -2721->162.5-41NA
    Micafungin 489, 49236M38-AMIC-1, -2721->325.252->16>16
188 3XTT colorMIC-2240.25-128NA32NA
  • a M38-A, CLSI M38-A reference method for broth dilution antifungal susceptibility testing of filamentous fungi (307). M38-A-like, method which modifies the M38-A reference method.

  • b ”Red well” and “purple well,” endpoints used in colorimetric assays. MIC-0, -1, and -2, endpoints by visual inspection, where MIC-0 is optical clearance, MIC-1 is +1 turbidity, and MIC-2 is +2 turbidity.

  • c NA, not available.