TABLE 5.

Published real-time PCR assays for E. histolytica and E. dispar

AssayGene targetAmplicon (bp)Primer or probeSequence (5′→3′)Nucleotide positionReference(s)
Light cycler18S rRNA307Eh-S26CaGTACAAAATGGCCAATTCATTCAACG190-21618, 27, 139
Ed-27 CbGTACAAAGTGGCCAATTTATGTAAGCA191-217
Eh-Ed-AS25cGAATTGATTTTACTCAACTCTAGAG497-473
Eh/Ed-24LC-Red 640LC-Red-640-TCGAACCCCAATTCCTCGTTATCCp373-350
Eh-Ed-25-FcFL-GCCATCTGTAAAGCTCCCTCTCCGAX400-376
TaqMan 118S rRNA231Eh-d-239FaATTGTCGTGGCATCCTAACTCA260-23998, 139, 195, 198
Ehd-88RbGCGGACGGCTCATTATAACA88-107
Histolytica-96TaVIC-TCATTGAATGAATTGGCCATTT- nonfluorescent quencher217-197
Dispar-96TbFAM-TTACTTACATAAATTGGCCACTTTG-nonfluorescent quencher218-194
TaqMan 2Episomal repeats (SREPH gene)83Histolytica-50FaCATTAAAAATGGTGAGGTTCTTAGGAA50-76139, 198, 200
Histolytica-132RaTGGTCGTCGTCTAGGCAAAATATT132-109
Histolytica-78TaFAM-TTGACCAATTTACACCGTTGATTTTCGGA-Eclipse Dark quencher106-78
137Dispar-1FbGGATCCTCCAAAAAATAAAGTTTTATCA1-28
Dispar-137RbATCCACAGAACGATATTGGATACCTAGTA137-109
Dispar-33bHEX-UGGUGAGGUUGUAGCAGAGAUAUUAAUU-TAMRA33-60
Multiplex real time PCR18S rRNA172Ehd-239FcATTGTCGTGGCATCCTAACTCA260-239195
Ehd-88RcGCGGACGGCTCATTATAACA88-107
Histolytica-96TaVIC-TCATTGAATGAATTGGCCATTT- nonfluorescent quencher217-197
Artus (Hamburg, Germany) real-time LC-PCR kitd230E. histolyticaNDe55
SYBER green18S rRNA877PSP5aGGCCAATTCATTCAATGAATTGAG200-223139
PSP3aCTCAGATCTAGAAACAATGCTTCTC1076-1052
878NPSP5bGGCCAATTTATGTAAGTAAATTGAG200-224
NPSP3bCTTGGATTTAGAAACAATGTTTCTTC1077-1052
Molecular beacon18S rRNA134EhfaAACAGTAATAGTTTCTTTGGTTAGTAAAA104-238153
EhraCTTAGAATGTCATTTCTCAATTCAT
Molecular beacon probeTexas Red-GCGAGC-ATTAGTACAAAATGGCCAATTCATTCA-GCTCGC-dR Elle
  • a Specific for E. histolytica.

  • b Specific for E. dispar.

  • c Specific for E. histolytica and E. dispar.

  • d Discontinued.

  • e ND, not described.