TABLE 3.

Automated systems of nucleic acid extraction and purification for rapid real-time PCR assays

ManufacturerTechnologic principleaAutomated platformsSpecimen throughputWt (kg) and dimensions (W × D × H, cm)Specimen typeLiterature reference(s)
Roche Applied Science (www.Roche-Applied-Science.com ; www.magnapure.com )NA capture by magnetic silica particlesMagNA Pure LC MagNA Pure Compact32 samples in 90 min 8 samples in 30 min151; 90× 60 × 65 60; 54 × 61× 57Serum, plasma, whole blood, stool, respiratory tract specimesns, swabs (dermal, genital) 114, 116, 139, 185, 208, 210, 211, 213, 215, 223, 226, 245, 253, 255, 270, 287, 310, 506
Qiagen (www.qiagen.com )NA capture by magnetic silica particlesBioRobot EZ16 samples in 20 min26; 28.2 × 45.5 × 43Swabs (dermal, genital, nasal) 116,>226
BioRobot M4848 samples in 159 min130; 107 × 60 × 87.5
BioRobot M9696 samples in 221 min130; 107 × 65× 77.9
BioRobot 960496 samples in 2 h95; 120 × 71 × 60
BioRobot MDx96 samples in 2.5 h140; 145 × 82 × 81
Applied Biosystems (www.appliedbiosystems.com )NA capture by silica fiber membrane under vacuum conditionsABI Prism 6100 ABI Prism 670096 samples in 30 min 96 samples in 75 min; 4 microtiter plates in 3.5 h20; 53 × 48 × 27 227; 142 × 84 × 99 208, 446
bioMérieux (www.biomerieux-usa.com )NA capture by silica bound to filter under vacuum conditionsNucliSens Extractor10 samples in 35-45 min90; Cylindrical (66 diam. × 79 ht.)Plasma 143
  • a NA, nucleic acid.