TABLE 8.

Comparison of LightCycler assays to culture-based assays for selected pathogensa

Organism (reference)Sensitivity increase for LightCycler (%)Analytical turnaround time
LightCyclerb (min)Culture-based assay (days)
Group A streptococci (13)7301-2
Legionella spp. (5) (BALc)0d452-14
Bordetella pertussis (6)219503-7
Vancomycin-resistant enterococci (fecal surveillance samples) (451)120e452-3
Varicella-zoster virus (skin) (117)91452-5
Herpes simplex virus (skin, genital) (115)23451-5
Cytomegalovirus (urine)88e45≤1
  • a Adapted and modified from reference 73 with kind permission of Springer Science and Business Media.

  • b The time shown is for the assay only and does not include the time required for extraction of nucleic acid, which may vary from minutes to over 1 hour depending on the specimen type, organism, and whether a manual or automated method is used.

  • c BAL, bronchoalveolar lavage.

  • d Only archived culture-positive samples were available for this comparison.

  • e Based on unpublished clinical studies at the Mayo Clinic, Rochester, Minn.