TABLE 1.

Instruments available

ManufacturerInstrumentProbe chemistries supportedExcitation (nm)Detection (nm)No. of samplesReaction volume (μl)Rapid thermocyclingComments
Roche Applied Science (http://www.roche-applied-science.com/lightcycler-online/ )LightCycler 1.0Hybridization probes, molecular beacons, TaqManLED470530, 640, 7103210-20YesSpecial sample containers.
LightCycler 2.0Hybridization probes, molecular beacons, TaqManLED470530, 555, 610, 640, 670, 7103210-100YesSpecial sample containers.
Cepheid (http://www.cepheid.com/pages/home.html )SmartCycler IIMolecular beacons, TaqManLED450-495, LED500-550, LED565-590, LED630-650510-527, 565-590, 606-650, 670-7501625-100YesSpecial sample containers. Independent modules.
Corbett Research (http://www.corbettresearch.com/home.htm )Rotor-Gene 3000Molecular beacons, TaqManLED470, LED530, LED585, LED635510, 550, 580, 610, 6607210-150 5-50MidrangeStandard plastic tubes are used.
ABI (http://www.appliedbiosystems.com/index.cfm )Prism 7000TaqMan, molecular beaconsTungsten/halogen4-color multiplex96No
Prism 7300TaqMan, molecular beaconsTungsten/halogen3-color multiplex96No
Prism 7500TaqMan, molecular beaconsTungsten/halogen4-color multiplex96No
Prism 7900htTaqMan, molecular beaconsLaser 488500-660384No
BioRad (http://www.bio-rad.com/ )MyiQTaqMan, molecular beaconsTungsten/halogenSingle color9615-100No
iCycler iQTaqMan, molecular beacons, hybridization probesTungsten/halogen4-color multiplex9650No
Stratagene (http://www.stratagene.com/homepage/ )Mx4000TaqMan, molecular beaconsTungsten/halogen4-color multiplex9610-50No
Mx3000pTaqMan, molecular beaconsTungsten/halogen4-color multiplex9625No
MJ Research (http://www.mjr.com/ )Chromo 4TaqMan, molecular beacons450-490, 500-535, 555-585, 620-730515-530, 560-580, 610-650, 675-7309610-100No
OpticonTaqMan, molecular beacons450-495515-5459610-100No
Opticon 2TaqMan, molecular beacons470-505525-543, 540-7009610-100No
Genetic Discovery Technology (http://www.biogene.com/index.cfm )SynChronTaqMan, molecular beacons, hybridization probes473 laser520-720610-50YesElectrically conducting polymer technology.