TABLE 2.

Manual methods of nucleic acid extraction and purification for rapid real-time PCR assays

Kit (manufacturer)Technologic principleaSpecimen throughputSpecimen typeReferencesb
High Pure (Roche Applied Science; www.roche-applied-science.com )NA capture by glass fiber fleece immobilized in a special plastic filter tube and subjected to centrifugation24 samples in 1 hSerum, whole blood, plasma, urine, stool, sterile body fluids, respiratory tract specimens, swabs (genital, dermal) 14, 18, 100, 338, 401, 403
QIAamp (Qiagen; www.qiagen.com )NA capture by silica gel membrane placed in tube column and subjected to centrifugation or vacuum conditions24 samples in 1 h for DNA; 24 samples in 1.5 h for RNARespiratory tract specimens, plasma, stool, serum, whole blood, urine, sterile body fluids, swabs (nasal, fecal) 18, 19, 36, 120, 147, 148, 184, 193, 198, 210, 212, 214, 225, 226, 234, 235, 238-242, 244, 246, 247, 250, 254, 262, 264, 266, 269, 273, 277, 279, 282, 291, 294, 304, 323, 362, 401, 402, 484, 486, 494, 515, 530, 531
IsoQuick (Orca Research; www.bioexpress.com )NA is partitioned into an aqueous phase and then precipitated with ethanol and resuspended in water or buffer24 samples in 1 h for DNA; 24 samples in 2 h for RNAPlasma, whole blood, stool, respiratory tract specimens, sterile body fluids, swabs (dermal, fecal, genital) 115-118, 120, 184, 213, 256, 259-261, 264-266, 299, 399, 450, 451
IsoCode Stix (Schleichar & Schuell; www.whatman.com )DNA bound to Matrix and released by simple water and heat elutionProcessed individuallyWhole blood 561, A, B
  • a NA, nucleic acid.

  • b A, A. Muyombwe, I. Lundgren, L. M. Sloan, J. E. Rosenblatt, P. G. Kremsner, S. Borrmann, and S. Issifou, Program Abstr. 52nd Am. Soc. Trop. Med. Hyg., abstr. 744, 2003; B, J. E. Rosenblatt, A. Muyombwe, L. M. Sloan, P. Petmitr, and S. Looareesuman, Program Abstr. 11th Int. Cong. Infect. Dis., abstr. 14.006, 2004.