TABLE 5.

Sensitivities and specificities of diagnostic tests for melioidosis

TestaStudy location (n)Sensitivity (%)Specificity (%)Reference
Antibody detection
    Complement fixation testUnknown (47)Not recorded100314
    Serum IHAThailand (150)7175142
    Serum IHAThailand (184)779215
    Serum IHA (purified antigens)Thailand (101)46-9434-82386
    Serum IHAThailand (130)61.979.9375
    Serum IHAThailand (148)6493479
    Serum IHAThailand (299)7268490
    Serum IHAAustralia (298)8091319
    Serum IHA (acute-phase serum only)Australia (191)8510025
    Serum IFA (IgG, acute-phase serum only)Australia (191)869925
    Serum ELISA (IgG; acute-phase serum only)Australia (191)799925
    Serum ELISA (purified glycolipid antigen)Japan, Vietnam, Thailand (416)10097.8330
    Serum ELISA (IgG; immunoaffinity-purified antigen)Thailand (150)8883142
    Serum ELISA (IgM)Thailand (150)87.881.8142
    Serum ELISA (various antigens)Thailand (101)74-8275-80386
    Serum ELISA (immunoaffinity-purified antigen)Thailand (130)71.486.2375
    Serum ELISA (culture filtrate antigen)Thailand (148)9397479
    Serum DOT (culture filtrate antigen)Thailand (101)7264386
    Serum DOT (culture filtrate antigen)Thailand (130)85.785.3375
    Serum DOT (culture filtrate antigen)Thailand (148)94.199.2479
    Immunochromogenic card test (IgG)Australia (298)8890319
    Immunochromogenic card test (IgM)Australia (298)7769319
    Immunochromogenic card test (IgG)Thailand (299)7990490
    Immunochromogenic card test (IgM)Thailand (299)6780490
    Serum (Western blot)Thailand (101)7091386
    Serum (various antibody targets)Thailand (101)23-5956-95386
    Serum IFA (whole-cell antigen)India (22)45-63291
Antigen detection
    Specimen immunofluoresenceThailand (272)7399462
    Serum ELISA (19.5-kDa antigen)Thailand (147)829614
    Direct specimen ELISA (MAb 5F8)Thailand (114)759810
    Urine IFAThailand8196136
    Blood PCR (LPS1, LPS2)Thailand (130)95.291.7375
    Blood PCR (16S rRNA)Australia (52)10067178
    Blood PCR (various primers)Thailand31-4147-100251
    Blood PCR (16S rRNA)Thailand (29)47100144
    Blood PCRThailand (7)100100352
Blood culture supernatant
    MAb-LAThailand (1, 369)95.199.713
    MAb-LAThailand10086335
    MAb-LAThailand10096335
    MAb-LAThailand (88)100100143
  • a IFA, immunofluorescence assay; DOT, DOT immunoassay; MAb-LA, monoclonal antibody latex agglutination.