TABLE 5.

Sensitivities and specificities of diagnostic tests for melioidosis

TestaStudy location (n)Sensitivity (%)Specificity (%)Reference
Antibody detection
    Complement fixation testUnknown (47)Not recorded100 314
    Serum IHAThailand (150)7175 142
    Serum IHAThailand (184)7792 15
    Serum IHA (purified antigens)Thailand (101)46-9434-82 386
    Serum IHAThailand (130)61.979.9 375
    Serum IHAThailand (148)6493 479
    Serum IHAThailand (299)7268 490
    Serum IHAAustralia (298)8091 319
    Serum IHA (acute-phase serum only)Australia (191)85100 25
    Serum IFA (IgG, acute-phase serum only)Australia (191)8699 25
    Serum ELISA (IgG; acute-phase serum only)Australia (191)7999 25
    Serum ELISA (purified glycolipid antigen)Japan, Vietnam, Thailand (416)10097.8 330
    Serum ELISA (IgG; immunoaffinity-purified antigen)Thailand (150)8883 142
    Serum ELISA (IgM)Thailand (150)87.881.8 142
    Serum ELISA (various antigens)Thailand (101)74-8275-80 386
    Serum ELISA (immunoaffinity-purified antigen)Thailand (130)71.486.2 375
    Serum ELISA (culture filtrate antigen)Thailand (148)9397 479
    Serum DOT (culture filtrate antigen)Thailand (101)7264 386
    Serum DOT (culture filtrate antigen)Thailand (130)85.785.3 375
    Serum DOT (culture filtrate antigen)Thailand (148)94.199.2 479
    Immunochromogenic card test (IgG)Australia (298)8890 319
    Immunochromogenic card test (IgM)Australia (298)7769 319
    Immunochromogenic card test (IgG)Thailand (299)7990 490
    Immunochromogenic card test (IgM)Thailand (299)6780 490
    Serum (Western blot)Thailand (101)7091 386
    Serum (various antibody targets)Thailand (101)23-5956-95 386
    Serum IFA (whole-cell antigen)India (22)45-63 291
Antigen detection
    Specimen immunofluoresenceThailand (272)7399 462
    Serum ELISA (19.5-kDa antigen)Thailand (147)8296 14
    Direct specimen ELISA (MAb 5F8)Thailand (114)7598 10
    Urine IFAThailand8196 136
    Blood PCR (LPS1, LPS2)Thailand (130)95.291.7 375
    Blood PCR (16S rRNA)Australia (52)10067 178
    Blood PCR (various primers)Thailand31-4147-100 251
    Blood PCR (16S rRNA)Thailand (29)47100 144
    Blood PCRThailand (7)100100 352
Blood culture supernatant
    MAb-LAThailand (1, 369)95.199.7 13
    MAb-LAThailand10086 335
    MAb-LAThailand10096 335
    MAb-LAThailand (88)100100 143
  • a IFA, immunofluorescence assay; DOT, DOT immunoassay; MAb-LA, monoclonal antibody latex agglutination.