TABLE 3.

Sensitivities and specificities of PCR assays for detection of B. burgdorferi DNA in different clinical specimens from patients with LBa

Clinical specimen and regionNo. of studies includedMedian % sensitivityb (range)Reported % specificity rangeReferences
Skin biopsy
    EM1669 (36-88)98-100
        United States464 (59-67)98-100 177, 301, 303, 327
        Europe1273 (36-88)100 31, 165, 209-211, 234, 256, 272, 279, 344, 358, 359
    ACA, Europe876 (54-100)100 31, 199, 209, 210, 256, 279, 344, 359
Blood, plasma, serum614 (0-100)
    United States318 (0-59)100 108, 172, 345
    Europe310 (4-100)NAc 78, 233, 234
CSF1638 (12-100)93-100
    United States673 (25-93)93-100 134, 150, 172, 180, 223, 239
    Europe1023 (12-100)98-100 11, 57, 74, 88, 130, 162, 233, 236, 270, 377
Synovial fluid878 (42-100)100
    United States483 (76-100)100 30, 172, 224, 252
    Europe466 (42-85)100 87, 133, 270, 293
  • a Only studies published in MEDLINE-indexed periodicals during the years 1991 to 2003 and those examined by PCR assay for ≥5 cases are included.

  • b Median sensitivity of PCR assays based on included studies. For studies tested with multiple PCR primer sets, the highest sensitivity reported was selected for analysis.

  • c NA, not available.