TABLE 1.

Deletion or insertion of Brucella genes resulting in attenuated bacteria

GeneFunctionSpeciesVirulenceReferences
In vitroIn vivo
pgm Phosphoglucomutase (LPS synthesis) B. abortus VirulentAttenuated 156
rfbE Perosamine synthetase (LPS synthesis) B. melitensis VirulentAttenuated 65
pmm Phosphomannomutase (LPS synthesis) B. abortus AttenuatedAttenuated 2
wboA Glycosyltransferase (LPS synthesis) B. abortus NDaAttenuated 104
cydB Cytochrome bd oxidase (respiratory system) B. abortus AttenuatedAttenuated 54
cgs Cyclic β-1,2 glucan synthetase B. abortus Attenuated (only S19)Attenuated (only S19) 23, 77
sod Cu-Zn SOD B. abortus VirulentAttenuated (only S2308) 154
dnaK Heat shock protein B. suis AttenuatedNDa 93
lon Protease B. abortus AttenuatedVirulent 135
bacA Cytoplasmic membrane transport protein B. abortus AttenuatedAttenuated 98
hfq Host factor-1, RNA-binding protein regulating stationary-phase stress resistance B. abortus AttenuatedAttenuated 136
virB Type IV secretion system B. suis, B. abortus AttenuatedAttenuated 59, 75, 116, 146
bvrRS Two-component regulatory protein B. abortus AttenuatedAttenuated 147
aroC Chorismate synthase (aromatic compound synthesis) B. suis, B. abortus AttenuatedAttenuated 59, 60, 75
purE De novo purine synthesis pathway B. melitensis AttenuatedAttenuated 41, 49, 76
  • a ND, not determined.