TABLE 1.

Substrate profiles of aminoglycoside phosphotransferases

PhosphotransferaseSubstrate(s)
APH (3′)
    IKanamycin, neomycin, lividomycin, paromomycin, ribostamycin
    IIKanamycin, neomycin, butirosin, paromomycin, ribostamycin
    IIIKanamycin, neomycin, lividomycin, paromomycin, ribostamycin, butirosin, amikacin, isepamicin
    IVKanamycin, neomycin, butirosin, paromomycin, ribostamycin
    VNeomycin, paromomycin, ribostamycin
    VIKanamycin, neomycin, paromomycin, ribostamycin, butirosin amikacin, isepamicin
    VIIKanamycin, neomycin
APH(2′′)
    Ia (bifunctional enzyme)Kanamycin, gentamicin, tobramycin, sisomicin, dibekacin
    Ib, IdKanamycin, gentamicin, tobramycin, netilmicin, dibekacin
    IcKanamycin, gentamicin, tobramycin
APH(3′′)-Ia, -IbStreptomycin
APH(7′′)-IaHygromycin
APH(4)-Ia, -IbHygromycin
APH(6)-Ia, -Ib, -Ic, -IdStreptomycin
APH(9)-Ia, -IbSpectinomycin