TABLE 1.

Methods of isolation of Y. enterocolitica most commonly used for clinical, food, and environmental samples

PreenrichmentaSelective enrichmentaPostenrichmentSelective agar plateSerotype(s) recoveredReference(s)
PSB, 4°C, 3-4 wkMAC, 25°C, 48 h, CIN, 30°C, 24 hAll99, 112a
PSB, 10°C, 10 daysKOHCIN and MAC, 22-26°C, 48 hAll39
PBS, 4°C, 21 daysCIN, 30°C, 24-48 hAll4
PBS/PSB, 25°C, 1-3 daysKOHMAC, 25°C, 48 h; CIN, 30°C, 24 hAll35, 68
SEL, 22°C, 3 daysMAC, 25°C, 48 hO:3, O:891
PSB, 4°C, 8 daysMRB, 22°C, 4 daysCIN, 30°C, 24 hO:3, O:9112a, 130
YER, 4°C, 9 daysBOS, 22°C, 5 daysCIN, 30°C, 24 hO:3, O:8130
TSB, 22°C, 1 dayBOS, 22°C, 7 daysCIN, 30°C, 24 hO:3, O:8131
ITC, 25°C, 2 daysSSDC, 30°C, 24 hO:368, 158
  • a SEL, selenite broth; YER, yeast extract-rosebengal broth.