TABLE 10.

Studies examining the humoral immune responses to Malassezia of patients with SD and dandruff and of controls

ReferenceSubjectsMethodaAntigenic preparation and strain usedResults
1224 dandruff, 24 hair loss, 10 controlsIIFWhole cells of P. ovale and P. orbiculareHighest titers seen in patients with largest amount of dandruff; similar titers for both organisms
29240 SD, 40 controlsCIECytoplasmic antigens from several isolates of unknown origin73% of SD patients had antibodies compared to 25% of controls (P < 0.001)
ELISACytoplasmic antigens of P. orbiculare, unknown originMean level of antibodies higher in patients due to IgG but not IgM
4930 SD, 60 controlsIIFP. ovale (ATCC 42132) whole cellsNo differences in titers of IgG between patients and controls
5010 SD, 10 controlsIIF, FACS, ELISAP. ovale (ATCC 42132) (whole cells, protein extract, and carbohydrate extract)The only method able to demonstrate differences in titers between patients and controls was ELISA with the protein antigen (titers lower in patients than controls, P < 0.02)
5130 SDIIFP. ovale (ATCC 42132) whole cellsNo differences in levels of IgG in patients compared to historical controls
21919 SD, 19 controlsIIFP. ovale (ATCC 42132) whole cellsNo differences in titers of IgG between patients and controls
2510 SD, 10 controlsELISAWhole cells of M. furfur serovars A, B, and CNo differences in levels of Igs, IgM, IgG, IgG1–IgG4, or IgA between patients and controls
39925 SD, 10 controlsELISAM. furfur clinical isolates (oval, round, and elliptical forms), dialyzed culture medium (exoantigens)Titers of IgG higher in patients than in controls; titers of IgM higher in patients than controls (to oval form only); no differences in titers of IgA between patients and controls
32819 SD, 19 controlsELISAM. furfur L251 and GM 812 (whole cells and cytoplasmic preparation)No differences in titers of IgG or IgM to either antigen between patients and controls
  • a CIE, Countercurrent immunoelectrophoresis; FACS, fluorescence activated cell sorter analysis.