TABLE 5.

Description of PCR and extraction methods for detection of C. pneumoniae in vascular tissuea

StudyYrDNA extractionPCR method 1Target genePCR method 2Target genePCR method 3Target genePCR method 4Target gene
Kuo et al. (68)1993IHS + HCloned PstINDb16S rRNA
Kuo et al. (73)1995IH or QiagenS + HCloned PstIND
Campbell et al. (17)1995IH or QiagenS + HCloned PstIND
Grayston et al. (43)1995IH or QiagenS + HCloned PstIND
Ong et al. (102)1996In houseN + HMOMP codingND
Weiss et al. (144)1996In houseS + EIA16S rRNAND
Blasi et al. (9)1996In houseN16S rRNAND
Ramirez et al. (113)1996nsS16S rRNAS + EIA16S rRNAS + HCloned PstIMOMP codingNS
Juvonen et al. (65)1997IH + QiagenS + HMOMP codingND
Juvonen et al. (66)1997IH + QiagenS + HMOMP codingND
Jackson et al. (58)1997IHS + HCloned PstIND
Jackson et al. (59)1997IHS + HCloned PstIND
Kuo et al. (74)1997IHS + HCloned PstIND
Maass et al. (82)1997IHN + HCloned PstIND
Nyström-Rosander et al. (100)1997IHN16s rRNAND
Maass et al. (83)1998IHN + HCloned PstIND
Petersen et al. (112)1998QiagenNMOMP codingND
Lindholt et al. (80)1998IHN + TRFMOMP codingND
Paterson et al. (107)1998IHNMOMP codingND
Shor et al. (129)1998IHN + HMOMP codingND
Taylor-Robinson et al. (133)1998IHN + HMOMP codingND
Davidson et al. (25)1998IHS + HCloned PstIND
Andreasen et al. (3)1998IHS + H16S rRNAND
Ouchi et al. (104)1998IHN + RDCloned PstIND
Maass et al. (84)1998IHN + HCloned PstIND
Maass et al. (85)1998IHN + HCloned PstIND
Wong et al. (147)1999IHN + HMOMP codingND
Bartels et al. (6)1999IHN + HCloned PstIND
Nadrchal et al. (97)1999IHN16S rRNAND
Thomas et al. (138)1999IHN + HMOMP codingND
Blasi et al. (32)1999TriPureNCloned PstIRT-PCR16S rRNA
Jantos et al. (64)1999IH + QiagenS + H16S rRNANMOMP coding
Bartels et al. (7)2000IHN + HCloned PstIND
Berger et al. (8)2000IHQ-PCR16S rRNAND
Gibbs et al. (38)2000IHNCloned PstIND
Farsak et al. (33)2000IhS16S rRNAND
  • a S, single-step PCR; N, nested PCR; H, hybridization; IH, in house; RD, restriction enzyme digestion; RT-PCR, reverse transcriptase PCR.

  • b ND, not done.