TABLE 5.

Data on cellular immune responses to Malassezia in normal individuals used as control subjects

ReferenceNo. of subjectsAge range (yr)MethodAntigen preparationResults
9938–10LMIWhole yeast cell of Malassezia serovars A, B, and CPositive in all age groups; no difference in responses between age groups; responses generally higher to serovars B and CL
321–24LT
350–57
4091522–40LMIEther extraction, physical disruption, dialysis, and lyophilization of P. ovale and P. orbiculareMean LT response, 21–23a; Mean LMI response, 31.6%b
LT
4103220–42LT1. As above31 of 32 positive; mean LT response, 26.5a
2. Ether extraction, dialysis and lyophilization
3. Freeze-thaw, centrifugation
4. Sonication, centrifugation
282023–45LMIWhole yeast cell of Malassezia serovars A, B, and CLT, 3 of 20 positive; LMI, 8 of 19 positive; responses generally higher to serovars B and C
LT
461523–61LTFreeze-pressed M. furfur15, mean response of ≈31; 12, mean response of ≈27
1219–49
32816Mean, 56LT1. Whole cellsStimulation index obtained with the five antigens: 1, 0–30; 2, 0–50; 3, 0–5; 4, 0–7; 5, 0–12
2. Bead beater, centrifuged: cytoplasmic
3. Bead beater, ground pellet: cell wall
4. Sonicated, centrifuged: sonicate
5. Commercial preparationc
  • a Significant response, >4.

  • b Significant response, >20%.

  • c Commercial antigen preparation made by ALK, Copenhagen, Denmark.