TABLE 2.

Diagnostic value of different assays for prenatal diagnosis of congenital infection in 88 fetuses of 86 mothers with primary HCMV infection in pregnancya

Clinical specimenHCMV assayTest resultNo. of fetusesSensitivity (%)Specificity (%)PPV (%)NPV (%)
InfectedUninfected
Fetal bloodAntigenemiaNeg133563.910010072.9
Pos230
ViremiaNeg173141.410010064.6
Pos120
DNAemiaNeg52784.810010084.4
Pos280
Immediate-early mRNANeg21184.610010084.6
Pos110
pp67 mRNANeg41163.610010073.3
Pos70
IgM antibodyNeg153657.110010070.6
Pos200
Amniotic fluidVirus isolationNeg84481.810010084.6
Pos360
DNANeg33892.710010092.7
Pos380
Immediate-early mRNANeg23292.6979694.1
Pos251
pp67 mRNANeg23392.010010094.3
Pos230
  • a neg, negative; pos, positive; PPV, positive predictive value; NPV, negative predictive value. Source: M. G. Revello and G. Gerna, unpublished data.