TABLE 5.

Complex growth medium BM-3 for B. mandrillarisa

ComponentAmt
Basal medium
    Biosate peptone2.0 g
    Yeast extract2.0 g
    Torula yeast RNA0.5 g
    Double-distilled water345.0 ml
Supplements
    Hanks' balanced salts, 10×34.0 ml
    5% Liver digest in Hanks' salts100.0 ml
    MEM vitamin mixture, 100×5.0 ml
    Lipid mixture, 1,000× (Gibco)0.5 ml
    MEM nonessential amino acids, 100×5.0 ml
    10% Glucose5.0 ml
    Hemin, 2 mg/ml0.5 ml
    0.5% Taurine5.0 ml
    Calf serum50.0 ml
  • a Medium BM-3 was described previously in reference 66. pH adjusted to 7.2.