TABLE 1.

Undefined medium SCGYEM for cultivation of N. fowleria

ComponentAmt or concn
Organic
    Isoelectric casein10.0 g
    Glucose2.5 g
    Yeast extract5.0 g
    Fetal calf serum100.0 ml
    Penicillin-streptomycin200.0 μg/ml (each)
    Distilled water900.0 ml
Buffering
    Na2HPO41.325 g
    KH2PO40.8 g
  • a Medium SCGYEM was described previously by Chang (13) and De Jonckheere (21).