TABLE 2.

Detection of Legionella nucleic acid by gene probe and PCR in clinical samples

Clinical sample testedGene target(s)ResultsReference
Frozen respiratory secretionsrRNA (Gen-Probe)112 culture-positive, 63 positive; 230 culture-negative, 228 negative 78
Prospective respiratory secretionsrRNA (Gen-Probe)427 samples; compared to DFA, 63% sensitive, 95% specific 222
Respiratory secretionsrRNA (Gen-Probe)167 patients; 31-67% sensitive, 99% specific 94
BAL mip gene68 samples, 15 positive 151
BAL5S rRNA, mip gene52 samples, 3 positive (EnviroAmp kit) 161
Respiratory secretions5S rRNA, mip gene31 samples, 12 positive (EnviroAmp kit) 201
BAL16S rRNA gene51 samples, 7 positive 183
Serum mip gene5 patients, acute- and convalescent-phase sera positive 182
Urine5S rRNA gene21 pneumonia patients, 11 positive by PCR, 9 confirmed 193
BAL16S rRNA256 samples, 14 PCR positive 156
Intratracheal aspirates mip gene, 5S rRNA1 patient, 8 samples positive with both primers 169
Serum, urine5S rRNA gene28 patients, 18 positive 210
Serum, urine700-bp fragmentSerum, 12 of 41 samples positive; urine, 6 of 20 samples positive 206
Respiratory secretions (BAL, sputum, pleural fluid, lung tissue, bronchial washing)16S rRNA gene212 specimens; 31 of 31 culture-positive samples positive; 12 culture-negative samples positive by PCR, 4 confirmed by sequencing 51
BAL mip gene, 5S rRNA43 specimens (LightCycler) 131
Respiratory secretions (BAL, sputum)16S rRNA77 samples, 2 culture-positive, both PCR-positive (LightCycler)