TABLE 4.

Defined medium M-11 for A. culbertsonia

ComponentConcn
Amino acids (mg/liter)
    l-Arginine · HCl825.0
    Glycine1,500.0
    l-Histidine · HCl160.0
    l-Isoleucine600.0
    l-Leucine900.0
    l-Lysine · HCl1,250.0
    l-Methionine300.0
    l-Phenylalanine900.0
    l-Threonine500.0
    l-Tryptophan200.0
    l-Valine700.0
Other (g/liter)
    Glucose1.8
    Na citrate1.0
Vitamins (mg/liter)
    Biotin0.25
    B120.00125
    Thiamine · HCl1.25
Salts (g/liter)
    CaCl2 · 2H2O0.0588
    MgSO4 · 7H2O0.985
    KH2PO40.340
    Na2HPO4 · 2H2O0.445
    (NH4)2SO4 · FeSO4.6H2O0.0196
Trace elements (mg/liter)
    MnCl2 · 4H2O2.3
    ZnSO4 · 7H2O1.0
    (NH4) Mo7O24.4H2O0.4
    CuSO4 · 5H2O0.0033
    CoCl20.017
    H3BO30.1
    Na2EDTA0.01
  • a Medium M-11 was described previously by Shukla et al. (69). Initial pH, 6.5.