TABLE 2.

Defined medium for pathogenic Naegleria spp.a

ComponentConcn (mg/liter)
Amino acids
    l-Alanine460.0
    l-Asparagine600.0
    l-Aspartic acid1,170.0
    l-Arginine940.0
    l-Cysteine · HCl40.0
    l-Glutamic acid1,865.0
    l-Glutamine600.0
    Glycine2,705.0
    l-Histidine200.0
    l-Isoleucine525.0
    l-Leucine760.0
    l-Lysine · HCl880.0
    l-Methionine970.0
    l-Phenylalanine480.0
    l-Proline1,200.0
    dl-Serine600.0
    l-Threonine530.0
    l-Tryptophan140.0
    l-Tyrosine180.0
    l-Valine700.0
Nucleic acid precursors
    Adenine10.0
    Adenosine 3′,2′-monophosphoric acid15.0
    Guanosine10.0
    Guanosine 3′,2′-monophosphoric acid10.0
    Hypoxanthine10.0
    Cytosine · H2O10.0
    Cytidine 3′,2′-monophosphoric acid25.0
    Thymine10.0
    Thymidine16.0
    Uracil10.0
    Uridine 3′,2′-monophosphoric acid15.0
Vitamins
    p-Aminobenzoic acid1.0
    Biopterin0.01
    d-Biotin0.01
    Choline chloride1.0
    Folic acid1.0
    Hemin10.0
    i-Inositol1.0
    Nicotinamide1.0
    Ca pantothenate1.0
    Pyridoxal · HCl1.0
    Pyridoxamine · diHCI1.0
    Riboflavin1.0
    Thiamine1.0
    dl-Thioctic acid0.5
    B120.001
Salts and metals
    CaCl240.0
    MgSO4 · 7H2O800.0
    KH2PO4362.0
    Na2HPO4500.0
    FeCl2 · 4H2O0.0105
    FeSO4 · 7H2O0.0105
    MnSO4 · H2O0.0095
    ZnSO4 · 7H2O0.0220
    (NH4)Mo7O24 · 4H2O0.0036
    CuSO4 · 5H2O0.0016
    NH4VO30.00046
    CoSO4 · 7H2O0.00048
    H3BO30.00057
    NiSO4 · 6H2O0.00045
    CrK(SO4) · 12H2O0.00096
    BaCl2 · 2H2O0.00050
Other
    d-Glucose8,000.0
    Na citrate100.0
    l-Ornithine.HCl40.0
    Putrescine.diHCl1.0
    Orotic acid20.0
    dl-Citruline8.0
    5-Sulfosalicylic acid1.0
  • a This defined medium was described previously by Nerad et al. (58).