TABLE 4.

Cell lines, media, and supplements that have been used to cultivate human-infecting opportunistic microsporidia

SpeciesCell linesaMediumSupplements and culture conditionsb
Encephalitozoon cuniculi E6, HLF, MDCK, MRC-5EMEM + 2 mM glutamine5 or 10% FBS or FCS and antibiotics (penicillin and streptomycin or gentamicin and amphotericin B), with or without 5% CO2
Encephalitozoon hellem MDCK, E6, HLF, MRC-5, RK-13, FBFEMEM + 2 mM glutamine, RPMI5 or 10% FBS or FCS and antibiotics (penicillin and streptomycin or gentamicin, amphotericin B), with or without 5% CO2
Encephalitozoon intestinalis E6, HLF, MDCK, HEL, MDM, RK-13, I047, HT-29, Caco-2EMEM + 2 mM glutamine, RPMI 1640, DMEM + 2 mM glutamine5 or 10% FBS, 5 or 10% FBS or FCS, antibiotics (penicillin and streptomycin or gentamicin or a cocktail of amoxicillin, vancomycin, gentamicin, and flucytosine, amphotericin B), with or without 5% CO2
Enterocytozoon bieneusi E6, HLF, HT-29, Caco-2, MDCK, RK-13EMEM + 2 mM glutamine5 or 10% FBS, 5% or 10% FBS, EGF, transferrin, insulin, HC, selenium, HEPES
Vittaforma corneae SIRC, MDCK, E6, HLF, MRC-5EMEM + 2 mM glutamine5% or 10% FBS, gentamicin, with or without 5% CO2
Trachipleistophora hominis MDCK, RK-13, COS-1, L6-C10, MMDMEM + 2 mM glutamine10% FBS, 10% HS, gentamicin, with or without 5% CO2
Brachiola algerae E6, HLFEMEM + 2 mM glutamine5 or 10% FBS, gentamicin and amphotericin B, without 5% CO2
  • a E6, monkey kidney; HLF, human lung fibroblasts; MDCK, Madin-Darby canine kidney; MRC-5, lung fibroblasts; RK-13, rabbit kidney; FBF, fetal bovine lung fibroblasts; HEL, human embryonic lung; MDM, monocytes; I 047, intestinal; HT-29, human adenocarcinoma; Caco-2, human colon carcinoma; SIRC, rabbit corneal epithelium; COS-1, green monkey kidney; L6-C10, human cell line; MM, mouse myoblasts.

  • b FCS, fetal calf serum; EGF, epidermal growth factor; HC, hydrocortisone; HS, human serum.