Table 2.

Comparison of the putative mycobacterial insertion element transposase genes belonging to the IS110 family

Insertion sequenceMycobacterial speciesAccession no.Nucleotide sequence length (bp) of:% Sequence similarity to IS900 (no. of nucleotides)cSequence length of transposase (amino acids)% Sequence similarity to IS900 (no. of amino acids)d
Insertion sequenceaTransposaseb
IS900M. paratuberculosisX162931,4511,200399
IS901M. aviumX592721,4721,20673% (232)40151% (212)
IS902M. silvaticumX580301,4701,20373% (233)40052% (213)
IS1110M. aviumZ230031,4621,39574% (113)46451% (189)
IS1547M. tuberculosisY134701,351∼1,182NSe∼380f31% (118)
IS1626M. aviumAF0710671,4181,179g82% (859)39366% (272)
  • a Nucleotide sequence length of the entire insertion element (107, 191).

  • b Nucleotide sequence length of the structural gene encoding the putative transposase for each insertion element.

  • c Nucleotide sequence similarities were determined using BLAST version BLASTN 2.0.9 (National Center for Biotechnology Information databases).

  • d Amino acid sequence similarities were determined using BLAST version BLASTP 2.0.9 (National Center for Biotechnology Information databases).

  • e NS, no significant similarity.

  • f Nucleotide and amino acid sequence lengths of putative transposase are variable, since the stop codon is not within the IS147 sequence (107).

  • g The function of ORF1179 is unknown (244).