Table 2.

Comparison of the putative mycobacterial insertion element transposase genes belonging to the IS110 family

Insertion sequenceMycobacterial speciesAccession no.Nucleotide sequence length (bp) of:% Sequence similarity to IS900 (no. of nucleotides)cSequence length of transposase (amino acids)% Sequence similarity to IS900 (no. of amino acids)d
Insertion sequenceaTransposaseb
IS900 M. paratuberculosis X16293 1,4511,200399
IS901 M. avium X59272 1,4721,20673% (232)40151% (212)
IS902 M. silvaticum X58030 1,4701,20373% (233)40052% (213)
IS1110 M. avium Z23003 1,4621,39574% (113)46451% (189)
IS1547 M. tuberculosis Y13470 1,351∼1,182NSe ∼380f 31% (118)
IS1626 M. avium AF071067 1,4181,179g 82% (859)39366% (272)
  • a Nucleotide sequence length of the entire insertion element (107, 191).

  • b Nucleotide sequence length of the structural gene encoding the putative transposase for each insertion element.

  • c Nucleotide sequence similarities were determined using BLAST version BLASTN 2.0.9 (National Center for Biotechnology Information databases).

  • d Amino acid sequence similarities were determined using BLAST version BLASTP 2.0.9 (National Center for Biotechnology Information databases).

  • e NS, no significant similarity.

  • f Nucleotide and amino acid sequence lengths of putative transposase are variable, since the stop codon is not within the IS147 sequence (107).

  • g The function of ORF1179 is unknown (244).