Table 8.

Summary of myosin and heart cross-reactive T-cell epitopes of streptococcal M5 proteina

Peptide (amino acids)SequencebOrigin of T-cell clone or response (reference)c
1–25TVTRGTISDPQRAKEALDKYELENHARF/valve (216)
81–96DKLKQQRDTLSTQKETLEREVQNARF/valve (216)
163–177ETIGTLKKILDETVKARF/valve (216)
337–356LRRDLDASREAKKQVEKALNormal/PBL (435)
347–366AKKQVEKALEEANSKLAALEMice (436)/normal PBL (435)
397–416LKEQLAKQAEELAKLRAGKAARF/PBL (435)
NT4 (40–58)GLKTENEGLKTENEGLKTEBALB/c lymph noded
NT5 (59–76)KKEHEAENDKLKQQRDTLBALB/c lymph noded
B1B2 (137–154)VKDKIAKEQENKETIGTLBALB/c lymph noded
B2 (150–167)TIGTLKKILDETVKDKIABALB/c lymph noded
C2A (254–271)EASRKGLRRDLDASREAKBALB/c lymph noded
C3 (293–308)KGLRRDLDASREAKKQBALB/c lymph noded
  • a Reprinted from reference123 with permission from the publisher.

  • b The amino-terminal TVTRGTIS sequence was taken from the M5 amino acid sequence published by Manjula et al. (342) and deviates from the M5 sequence published by Miller et al. (361) at positions 1 and 8. Other sequences (81–96 and 163–177) were taken from the PepM5 sequence reported by Manjula (342). These two sequences are found in the sequence as 67 to 89 and 174 to 188, respectively, as reported by Miller et al. (361). All other sequences shown are from the M5 gene sequence reported by Miller et al. (361). Underlined sequences are those shared among the epitopes or sequences compared in Table 2.

  • c PBL, peripheral blood lymphocytes; ARF, acute rheumatic fever.

  • d BALB/c mice were immunized with purified human cardiac myosin, and the recovered lymph node lymphocytes were stimulated with each of the peptides in tritiated-thymidine uptake assays (123).