Table 2.

Fungal phospholipases

FungusEnzymeGene cloned (name)Substrate specificityaProtein size (amino acid residues)Molecular mass (kDa)Potential GPI anchor sequence for attachmentReference(s)
Pathogenic
Candida albicansPLB1Yes (caPLB1)PC, 1-palmitoyl-Lyso-PC, 1-arachidonyl-Lyso-PC, 1-olejoyl-Lyso-PC, f1-Palmitoyl-Lyso-PE, 1-Palmitoyl-Lyso-PI60584No101, 122, 193
PLB2Yes (caPLB2)60867bNo188
PLDYes (caPLD)1,710196.4b87
Candida glabrataPLBNoPC, Lyso-PCClancy et al., Programs Abstr. 36th Annu. Meet. Infect. Dis. Soc. Am., abstr. 316 and 317
Cryptococcus neoformansPLBYes (PLB)PC61723, 24; Gottfredsson et al., Abstr. 97th Gen. Meet. Am. Soc. Microbiol. 1997
Aspergillus fumigatusPLBNoPC16; Koul et al., Abstr. 98th Gen. Meet. Am. Soc. Microbiol. 1998
PLCNo
PLDNo16
Aspergillus flavusPLBNoPC, Lyso-PC16; Koul et al., Abstr. 98th Gen. Meet. Am. Soc. Microbiol. 1998
PLANoUnknown
PLCNoUnknown
Nonpathogenic
Saccharomyces cerevisiaeYes (PLB1)e664Yes100, 223-225
PLBc200–280
PLB1d220
PLB2d14587, 206
PLDYes (SPO14)1,683195
Penicillium notatumPLBYes (PLB)PC and Lyso-PC60395Yes113, 163
Schizosaccharomyces pombeYesYes
Torulaspora delbrueckiiYes (PLB)Unknown64972Yes211
Neurospora crassaYesf
Kluyveromyces lactusYesf
  • a For abbreviations, see Table 1, footnote a.

  • b Predicted from the amino acid sequence and may vary due to posttranslational modifications.

  • c Secreted enzyme.

  • d Membrane-associated enzyme.

  • e Coding for a core protein moiety of the three isoforms of PLB present in S. cerevisiae.

  • f Gene sequence available in GenBank but not published.