Table 4.

Reagents and assays used in the detection of galactofuran-containing antigens in serum of patient with IA

AssayDetection limit (ng/ml)Reference(s)
Pretreatment of seruma
 Perchloric acid, room temperature603
 Citric acid, boiling12, 294, 639, 719-721
 TCA, boiling496, 573
 PBS, boiling177, 557, 728
 EDTA, boiling178, 205, 389, 395, 628
Type of Detector Antibody
 Polyclonal antibody2205
 MAb1628
Method of assay
 Latex15178, 242, 395, 690
 Radioimmunoassay7639, 721
 ELISA inhibition5345, 496, 573
 ELISA sandwich1628
 Ultrasound + latex + videomicroscopy0.1232
  • a TCA, trichloroacetic acid; PBS, phosphate-buffered saline.