Table 4.

Reagents and assays used in the detection of galactofuran-containing antigens in serum of patient with IA

AssayDetection limit (ng/ml)Reference(s)
Pretreatment of seruma
 Perchloric acid, room temperature 603
 Citric acid, boiling 12, 294, 639,719-721
 TCA, boiling 496,573
 PBS, boiling 177, 557,728
 EDTA, boiling 178, 205, 389, 395,628
Type of Detector Antibody
 Polyclonal antibody2 205
 MAb1 628
Method of assay
 Latex15 178, 242, 395, 690
 Radioimmunoassay7 639, 721
 ELISA inhibition5 345, 496, 573
 ELISA sandwich1 628
 Ultrasound + latex + videomicroscopy0.1 232
  • a TCA, trichloroacetic acid; PBS, phosphate-buffered saline.