Table 5.

Cytologic features of selected yeast genera

CriterionCharacteristic for:
Candida spp.Cryptococcusspp.Blastomyces spp.Histoplasmaspp.Coccidioides spp.
Size (μm)3–45–158–202–55–100 (mean, 10–40)
ShapeOvalRound-oval, clear capsuleSpherical, double-contoured wallOval, endocellularSpherical, refractile
BuddingBroad-based pseudohyphaeNarrow basedBroad basedSingle, narrowEndospores
PAP stainCyanophilicPale, cyanophilicBlue-greenDark outlineOrangeophilic
DQ stainDark blue-purpleBlue-purplePale blueDark blueColorless or light blue
Special stainGMS, PASaGMS, mucicarmine, alcian blueGMS, PASGMS, PASPAS, GMS
  • a PAS, Periodic acid-Schiff.