Table 3.

Staining characteristics of selected bacterial genera

GenusStaining witha:Other techniques
PAPRomanowskyH&EGramAcid fastSilver
Mycobacterium+++GMSPCR
(negative images)
Legionella +b+cDieterle, W-SDFA
modified
Actinomyces++++GMSDFA
Helicobacter++++Dieterle, W-S
Nocardia +b++dGMSDFA
  • a DFA, direct fluorescent antibody; W-S, Warthin-Starry; −, does not stain; +, visible with stain.

  • b Stains best with Giemsa.

  • c L. micdadei will stain acid-fast positive.

  • d Stains best with Fite’s silver stain.