Table 2.

Comparison of glycosidase and phosphatase tests used in systems for identification of gram-positive bacteria

EnzymeIdentification systema
Identifies Micrococcaceae andStreptococcaceaeIdentifiesMicrococcaceaeIdentifies Streptococcaceae
MS GPMS Rapid GPVitek GPIPasco GP IDAPI Staph IDGeary et al. (86)Kloos and Bannerman (129)API Staph ID 32StaphZymRapID STRRapid ID 32 StrepAPI 20 Strep
α-l-ArabinosidaseMEU
N-Acetyl-β-d-glucosaminidaseMEUPNP+
N-Acetyl-β-d-galactosaminidaseMEU
β-d-CellobiosidaseMEU
β-d-N′,N′-DiacetylchitobiosidaseMEU
α-d-GalactosidaseMEUPNP+6B
β-d-GalactosidasePNPMEUPNPPNP+++, +b βN
α-d-GlucosidaseMEUPNPPNP
β-d-GlucosidaseMEUPNPPNP++
β-d-GlucuronidasePNPMEUPNPPNPPNP+++AS
β-d-MannosidaseMEU+
PhosphatasePNP, IMEUPNPPNPPNPPNP++PNP+βN
No. of tests4120341434484
  • a For an explanation of the identification systems, see Table 1, footnote a. Synthetic moiety of substrates: AS, naphthol AS-BI; 6B, 6-bromo-β-naphthol; I, indoxyl; βN, β-naphthol; PNP, p-nitrophenol; +, proprietary synthetic moiety.

  • b Two substrates with different proprietary synthetic moieties.