Table 1.

Recovery of T. vaginalis from mouse vaginal washese

GroupaDose or treatmentaNo. of mice with T. vaginalis on the indicated day of culture/total no. inoculated
+7+14+21+28
14.5 × 105organisms/ml (n = 26)12/2610/25b8/25b,c7/25b,c
29 × 106 organisms/ml (n = 26)6/25b,c5/25b,c3/24b,c,d1/24b,c,d
31 × 108 organisms/ml (n = 26)6/25b,c4/23b,c1/21b,c,d0/21b,c,d
4PBS (n = 18)16/17b13/16b9/15b8/14b
5No immunization (n = 18)14/16b12/16b10/15b7/15b
6No treatment (negative control) (n = 6)0/60/60/60/6
  • a All mice in groups 1 to 5 received 0.05 ml of estradiol valerate subcutaneously on days −9 and −2, vaginal inoculation with 1010L. acidophilus organisms per ml on days −7 and −6, and vaginal infection with 2.5 × 107T. vaginalis organisms per ml. Groups 1 to 4 received subcutaneous inoculation of T. vaginalis on days −56 (in Freund’s complete adjuvant) and −28 (in Freund’s incomplete adjuvant) in the doses indicated. PBS, phosphate-buffered saline. Group 6 received none of the above manipulations.

  • b Total does not equal n because of unrelated death of mice.

  • c Statistically significant compared with group 4 value.

  • d Statistically significant compared with group 1 value.

  • e Reprinted from reference 3with permission of the publisher.