Platform | Read length (bp) | Output (Gb) | Coverage^{b} | Run time (h) | No. of reads | Cost per Gb ($) | Consumables cost ($) | Instrument cost ($) | Error rate | Dimensions (width × depth × ht) (cm) | Source(s) (reference[s]) |
---|---|---|---|---|---|---|---|---|---|---|---|

Sequencing by synthesis | |||||||||||

Illumina MiniSeq Mid Output | 2 × 150^{c} | 2.1–2.4^{c} | 8.6 | 17^{c} | 14 million–16 million^{c} | 2,584–2,953^{d} | 6,201^{c} | 55,411^{c} | 0.1% in >80% of base calls^{c} | 45.6 × 48 × 51.8^{c} | Illumina |

Illumina MiniSeq High Output | 1 × 75^{c} | 1.7–1.9^{c} | 6.8 | 7^{c} | 22 million–25 million^{c} | 3,264–3,648^{d} | 6,201^{c} | 55,411^{c} | 0.1% in >85% of base calls^{c} | 45.6 × 48 × 51.8^{c} | Illumina |

2 × 75^{c} | 3.3–3.8^{c} | 13.6 | 13^{c} | 44 million–50 million^{c} | 1,632–1,879^{d} | 6,201^{c} | 55,411^{c} | 0.1% in >85% of base calls^{c} | 45.6 × 48 × 51.8^{c} | Illumina | |

2 × 150^{c} | 6.6–7.5^{c} | 26.9 | 24^{c} | 44 million–50 million^{c} | 827–940^{d} | 6,201^{c} | 55,411^{c} | 0.1% in >80% of base calls^{c} | 45.6 × 48 × 51.8^{c} | Illumina | |

Illumina MiSeq Reagent kit v2 | 1 × 36^{c} | 0.54–0.61^{c} | 2.2 | 4^{c} | 12 million–15 million^{c} | 7,946–8,976^{d} | 4,847^{c} | 108,244^{c} | 0.1% in >90% of base calls^{c} | 68.6 × 56.5 × 52.3^{c} | Illumina |

2 × 25^{c} | 0.75–0.85^{c} | 3.1 | 5.5^{c} | 24 million–30 million^{c} | 5,702–6,463^{d} | 4,847^{c} | 108,244^{c} | 0.1% in >90% of base calls^{c} | 68.6 × 56.5 × 52.3^{c} | Illumina | |

2 × 150^{c} | 4.5–5.1^{c} | 18.3 | 24^{c} | 24 million–30 million^{c} | 950–1,077^{d} | 4,847^{c} | 108,244^{c} | 0.1% in >80% of base calls^{c} | 68.6 × 56.5 × 52.3^{c} | Illumina | |

2 × 250^{c} | 7.5–8.5^{c} | 30.5 | 39^{c} | 24 million–30 million^{c} | 570–646^{d} | 4,847^{c} | 108,244^{c} | 0.1% in >75% of base calls^{c} | 68.6 × 56.5 × 52.3^{c} | Illumina | |

Illumina MiSeq Reagent kit v3 | 2 × 75^{c} | 3.3–3.8^{c} | 13.6 | 21^{c} | 44 million–50 million^{c} | 1,362–1,568^{d} | 5,174^{c} | 108,244^{c} | 0.1% in >85% of base calls^{c} | 68.6 × 56.5 × 52.3^{c} | Illumina |

2 × 300^{c} | 13.2–15^{c} | 53.8 | 56^{c} | 44 million–50 million^{c} | 345–392^{d} | 5,174^{c} | 108,244^{c} | 0.1% in >70% of base calls^{c} | 68.6 × 56.5 × 52.3^{c} | Illumina | |

Illumina NextSeq 500 Mid Output | 2 × 75^{c} | 16.3–20^{c} | 71.8 | 15^{c} | <260 million^{c} | 318–391^{d} | 6,369^{c} | 266,835^{c} | 0.1% in >75% of base calls^{c} | 53.3 × 63.5 × 58.4^{c} | Illumina |

2 × 150^{c} | 32.5–39^{c} | 140 | 26^{c} | <260 million^{c} | 163–196^{d} | 6,369^{c} | 266,835^{c} | 0.1% in >80% of base calls^{c} | 53.3 × 63.5 × 58.4^{c} | Illumina | |

Illumina NextSeq 500 High Output | 1 × 75^{c} | 25–30^{c} | 107.7 | 11^{c} | <400 million^{c} | 312–374^{d} | 9,347^{c} | 266,835^{c} | 0.1% in >80% of base calls^{c} | 53.3 × 63.5 × 58.4^{c} | Illumina |

2 × 75^{c} | 50–60^{c} | 215.3 | 18^{c} | <800 million^{c} | 156–187^{d} | 9,347^{c} | 266,835^{c} | 0.1% in >80% of base calls^{c} | 53.3 × 63.5 × 58.4^{c} | Illumina | |

2 × 150^{c} | 100–120^{c} | 430.6 | 29^{c} | <800 million^{c} | 78–93^{d} | 9,347^{c} | 266,835^{c} | 0.1% in >75% of base calls^{c} | 53.3 × 63.5 × 58.4^{c} | Illumina | |

Single-molecule real-time sequencing | |||||||||||

Pacific Biosciences RS II P6-C4 chemistry | >20,000^{c} | 8–16^{e} | 57.4 | 0.5–4^{c} | 55,000^{c} | 250–500^{d} | 4,000^{e} | 695,000 | 14% errors per base | 203.0 × 90.0 × 160.0^{c} | PacBio, AllSeq^{f} (89) |

Pacific Biosciences Sequel system | >20,000^{c} | 80–160^{e} | 574.2 | 0.5–6^{c} | 370,000^{c} | 70–140^{d} | 11,200^{e} | 350,000 | 14% errors per base | 92.7 × 86.4 × 167.6^{c} | PacBio, AllSeq^{f} (89) |

Oxford Nanopore MinION Mk1 (1D) | >882,000 | 10–20^{c} | 71.8 | 1.67–72^{c} | 138,000 | 49.95–99.9^{d} | 999^{c} | 1,000^{c} | 12% errors per base | 10.5 × 3.3 × 2.3^{c} | Oxford Nanopore Technologies, Loman Labs^{g} (231) |

Oxford Nanopore MinION Mk1 (2D) | >882,000 | 10–20^{c} | 71.8 | 1.67–72^{c} | 138,000 | 49.95–99.9^{d} | 999^{c} | 1,000^{c} | 15% errors per base | 10.5 × 3.3 × 2.3^{c} | Oxford Nanopore Technologies, Loman Labs^{g} (231, 232) |

Oxford Nanopore PromethION single flow cell | <300,000^{c} | 233^{c} | 836.2 | 1.67–>72^{c} | 26 million^{c} | NA | NA | 135,000 (PEAP)^{c} | NA | 44.0 × 24.0 × 40.0^{c} | Oxford Nanopore Technologies |

Oxford Nanopore PromethION 48 flow cells | <300,000^{c} | 11,000^{c} | 3,9475.8 | 1.67–>72^{c} | 1.25 billion^{c} | NA | NA | 135,000 (PEAP)^{c} | NA | 44.0 × 24.0 × 40.0^{c} | Oxford Nanopore Technologies |

↵a All quantitative performance measures were taken from previously reported data, as indicated. Consumables costs were calculated as follows: Illumina costs included PhiX Control kit v3, the Nextera XT DNA sample preparation kit (96 samples)/Nextera DNA library preparation kit (96 samples), and Nextera XT Index kit v2 (96 indexes and 384 samples), the highest-output reagent kit. PEAP, PromethION Early-Access Program; NA, no data available.

↵b Calculated for 96 samples and the genome size of S. aureus strain MRSA252 (2,902,619 bp).

↵c Manufacturer's data.

↵d Estimated calculation for consumables.

↵e For 16 SMRT cells.

↵f See http://www.allseq.com/knowledge-bank/sequencing-platforms/pacific-biosciences/.

↵g See http://lab.loman.net/2017/03/09/ultrareads-for-nanopore/.