Table 2

Identification of Malassezia species from pure culture by sequencing and/or PCR-based methods a

PCR-based method and genomic regionOrigin(s) of strains and Malassezia speciesReference(s)
ITS amplification and sequencingCulture collection strain of M. furfur; clinical isolates of M. pachydermatis, M. restricta, M. dermatis, M. caprae, M. equina, M. cuniculi 39, 40, 194, 256, 257, 303
ITS amplification, REA, and sequencing and ITS and REA onlyType, neotype, culture collection strains, and clinical isolates of M. furfur, M. obtusa, M. globosa, M. slooffiae, M. sympodialis, M. restricta, M. pachydermatis, M. dermatis, M. japonica, M. nana, M. yamatoensis 111, 114, 286
26S rRNA gene (LSU) amplification and REA and 26S rRNA gene (LSU) amplification and sequencingClinical isolates of M. furfur, M. obtusa, M. globosa, M. slooffiae, M. sympodialis, M. restricta, M. pachydermatis, M. dermatis, M. caprae, M. equina, M. cuniculi M. japonica, M. nana, M. yamatoensis 39, 40, 47, 130, 137, 164, 223, 238
DNA microcoding array (Luminex xMAP platform) M. furfur, M. obtusa, M. globosa, M. slooffiae, M. sympodialis, M. restricta, M. pachydermatis, M. dermatis, M. japonica, M. nana, M. yamatoensis, M. equina 82
  • a ITS, internal transcribed spacer (ITS1-5.8S-ITS2) of the ribosomal DNA region; REA, restriction enzyme analysis; LSU, large subunit.