Table 5

Mutations in the cardiolipin synthetase gene, cls, identified among daptomycin-nonsusceptible Enterococcus strains

Species and mutationPredicted Cls domainDaptomycin MIC (μg/ml)Reference
Enterococcus faecium
    H215RProximal to the PLD1 active site (within 10 Å)48 a 164
    H270RSpacer regions between PLD1 and PLD232 157
    K60TLinker region32 160
    MPL insertion at 110Linker region24 a 164
    N13IN-terminal transmembrane helix32 a 164
    N13SN-terminal transmembrane helix192 157
    N13TN-terminal transmembrane helix>256 160
    R218QHKD of PLD132 a 164
    R218QHKD of PLD148 a 164
    R218QHKD of PLD116 162
Enterococcus faecalis
    Deletion of K61Linker region12 164
    R218QHKD of PLD1256 163
    N77-Q79 delLinker region256 163
    R218QHKD of PLD1512 163
  • a MIC measured by Etest.